 Tethering HJURP to a non-centromeric locus can give rise to a neocentromere, even after the disassociation of HJURP.There seems to be a co-evolutionary relationship between the "Drosophila" chaperone CAL1 and CENPA which accounts for species incompatibility—this is discussed more below.Whereas deposition of CENPA happens during S phase for "S. cerevisiae", two pathways of CENPA deposition in "S. pombe" determine when CENPA is deposited, namely S phase and G2.