 CENPA also been widely studied as an important player in centromere regulation as it binds and specifies centromere location for both normal centromeres and neocentromeres, regardless of DNA sequence.There is a general consensus that CENPA assembles into octameric nucleosomes, with two copies of CENPA replacing two copies of histone H3 at the target locus—this is the simplest model and much is still unknown about the composition of CENPA nucleosomes.Neocentromeres have been useful tools in investigating the effects of CENPA in the absence of satellite sequences.