 In order to make a conclusion about the clonality of a tumor, it is best to use the DNA from a normal tissue of the same person, and a sample without enzyme treatment must also be amplified as a control.If, even in normal tissues without enzyme treatment, a single band is observed, it may be explained as follows; this person has the genetic pattern as XO (this possibility can be excluded to see a band after enzyme treatment because, if indeed XO is the genetic pattern of the sample, then, there will be NO methylation, therefore no band should be visible after digesting with the enzyme.In the case of seeing a band after enzyme treatment, the observation is most likely to mean that the person has two X chromosomes with exact CAG repeats.)