 The first round was held 15 June 2016.Khon Kaen United was suspended from the 2016 campaign due to criminal case with 8 games remaining 

Following the death of King Bhumibol Adulyadej, the Football Association of Thailand cancelled the remaining matches and the four semi-finalists were awarded co-winners.A draw was held among them to select the team that will participate in 2017 AFC Champions League Play-off and was won by Sukhothai, while Chonburi withdrew from the draw.