 This includes but is not limited to trauma, sepsis, aseptic inflammation, myocardial infarction, stroke, transplantation, diabetes, and sickle cell disease.cfDNA is mostly a double-stranded extracellular molecule of DNA, consisting of small fragments (70 to 200 bp)  and larger fragments (21 kb)  and has been recognized as an accurate marker for the diagnosis of prostate cancer and breast cancer.Other publications confirm the origin of cfDNA from carcinomas and cfDNA occurs in patients with advanced cancer.