 When you see two bands for normal tissue (both enzyme treated and untreated), and you see two bands for enzyme treated tumor sample but two bands for untreated tumor DNA, then you are surely looking at a polyclonal tumor.However, if you see the same number of bands only with a single band after enzyme treatment, there is a rather high chance for the tumor of your interest to be monoclonal.In this last case, monoclonality is not for sure because, as stated earlier, there is the possibility of having exact same -CAG- repeats on both alleles.