 A cysteine-rich domain at the N-lobe of GPC3 has been identified to form a Wnt-binding hydrophobic groove including phenylalanine-41 that interacts with Wnt.Glypicans are expressed in various different amounts depending on the tissue, and they also are expressed to different degrees during the different stages of development.Drosophila Dally mutants have irregular wing, antenna, genitalia, and brain development.