 Methodology for the successful Chemical extraction of Acrasin was first described by Brian Shaffer in 1954 during his tenure at Princeton University in 1954.This involves the pouring of cold methanol over culture plates in which the aggregation of amoebas is general.This was dried in a vacuum at- 10 °C, and the residue was extracted with a small volume of methanol, which was then filtered and once again dried.