 In 1564, Ribault's former lieutenant René Goulaine de Laudonnière launched a second voyage to build a colony; he established Fort Caroline in what is now Jacksonville, Florida.War at home again precluded a resupply mission, and the colony struggled.In 1565 the Spanish decided to enforce their claim to "La Florida", and sent Pedro Menéndez de Avilés, who established the settlement of St. Augustine near Fort Caroline.