 TAP uses two types of agarose beads that bind to the protein of interest and that can be separated from the cell lysate by centrifugation, without disturbing, denaturing or contaminating the involved complexes.To enable the protein of interest to bind to the beads, it is tagged with a designed piece, the TAP tag.The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study.