 The L and U values – upper and lower limit of RF – give possibility to avoid the band region.formula_3

The last example of coefficient sensitive to minimal distance between spots is Retention distance (Komsta et al., 2007)

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The second group are criteria insensitive for minimal difference between RF values (if two compounds are not separated, such CRF functions will not indicate it).They are equal to zero in equal-spread state increase when situation is getting worse.