 1999, Bouveret et al., 2000, Puig et al., 2001).The first large-scale application of this technique was in 2002, in which the research team worked in collaboration with scientists of the proteomics company Cellzome to develop a visual map of the interaction of more than 230 multi-protein complexes in a yeast cell by systematically tagging the TAP tag to each protein.The first successful report of using TAP tag technology in plants came in 2004 (Rohila et al., 2004,)

There are a few methods in which the fusion protein can be introduced into the host cells.