 However, the method to be described assumes the use of a C-terminal tag, although the principle behind the method is still the same.TAP tagging was invented by a research team working in the European Molecular Biology Laboratory at late 1990s (Rigaut et al., 1999, Puig et al.,2001) and proposed as a new tool for proteome exploration.It was used by the team to characterize several protein complexes (Rigaut et al., 1999, Caspary et al.