"Regina Elena" remained in the active duty squadron through 1910, by which time her three sisters had been completed, bringing the total number of front-line battleships to six, including the two s.

On 29 September 1911, Italy declared war on the Ottoman Empire in order to seize Libya.For the duration of the conflict, "Regina Elena" was assigned to the 1st Division of the 1st Squadron along with her three sisters, under the command of Vice Admiral Augusto Aubry.