 Negative staining at both light microscope and electron microscope level should never be performed with infectious organisms unless stringent safety precautions are followed.Negative staining is usually a very mild preparation method and thus does not reduce the possibility of operator infection.Negative staining transmission electron microscopy has also been successfully employed for study and identification of aqueous lipid aggregates like lamellar liposomes (le), inverted spherical micelles (M) and inverted hexagonal HII cylindrical (H) phases (see figure).