 The specific way in which insulators block enhancers is dependent on the enhancers mode of action.Enhancers can directly interact with their target promoters through looping (direct-contact model), in which case an insulator prevents this interaction through the formation of a loop domain that separates the enhancer and promoter sites and prevents the promoter-enhancer loop from forming.An enhancer can also act on a promoter through a signal (tracking model of enhancer action).