 Lastly, the stain contrast and color is enhanced through the toning step with gold, in the form of gold chloride.This stain will appear as a dark brown color on parts of the nervous tissue such as the end-feet, myelinated fibers, and unmyelinated fibers.Bodian would go on in 1937 to refine the selectivity of the staining process through adjusting the formulations for the fixatives used.