 Like many printmakers of the period, he combined etching and engraving on the same plates, but in an unusually sensitive manner, exploiting the differences between the two techniques.He also engraved paintings by masters of the Renaissance, in particular Titian, whose very important destroyed altarpiece of the "Martyrdom of St. Peter Martyr" is now best known from Rota's engraving; he also made engravings after work by Michelangelo and Dürer.His known work includes more than one hundred and seventy engravings and etchings on a variety of subjects, including maps, vedutes, portraits, illustrations for pamphlets, coats of arms, and depictions of the saints.