 These lysine residues are located within the DNA-binding domain; acetylation inhibits the ability of FOXO1 to interact with the glucose-6 phosphatase promoter by decreasing the stability of the FOXO1-DNA complex.Additionally, this acetylation increases the rate of phosphorylation on Ser-253 by Akt.Mutating Ser-253 to Ala-253 makes FOXO1 constitutively active.