 A similar spectral shift to the longer monomeric peak is also observed when solvents like ethanol (or ethanol water mixture) is used as a vehicle or when nonionic detergents like Triton X-100 are used, that make exogenous micelles.Alcians blue carries Phthalocyanine one of the most highly colored chromophores yet known with a molar extinction of 120,000 i.e.Alcian blue is detectable at half the molar concentration of popular dyes like toluidine blue, tryarylmethanes (e.g.