 With the patient under some sort of anesthesia (depending on the rigidity of the scope), the fiber-optic cable is lowered into the correct area of the lower lungs (tracheobronchial tree), wedged into place, and saline applied.Once the saline is fully applied, then either suction is applied to collect the fluids, or the fluids are collected with a sterile syringe through the irrigation channel.The collection trap is then appropriately labeled and sent off for testing.