 Following John's death in 1494, the new king Manuel I of Portugal restored the freedom of the Jews.However, in 1497, under the pressure of the Spain through the marriage of Isabella, Princess of Asturias, the Church, and some Christians among the Portuguese people, King Manuel I of Portugal decreed that all Jews had to convert to Christianity or leave the country without their children.Hard times followed for the Portuguese Jews, with the massacre of 2000 individuals in Lisbon in 1506, further forced deportations to São Tomé (where there is still a Jewish presence today), and the later and even more relevant establishment of the Portuguese Inquisition in 1536.