 Hoechst and DAPI fluoresce brightly when bound to DNA, allowing them to serve as excellent nuclear stains.Microscope-based diagnostics are widely performed and served as a gold standard in histological analysis.However this procedure generally requires a series time-consuming lab-based procedures including fixation, paraffin embedment, sectioning, and staining to produce microscope slides with optically thin tissue slides (4-6 µm).