 The head office is based in Glasnevin, in Dublin.One of the founders of the Irish Wildlife Trust was well known Irish naturalist Eamonn de Buitléar

Similar to the UK based Wildlife Trusts the IWT is made up of local branches and volunteers, with branches in Dublin, Laois/Offaly, Galway, Cork, Waterford, Kerry, Longford/Westmeath and Cavan.However, the IWT sees itself as a single nationwide organisation rather than a federation of smaller, independent organisations though some separate conservation groups have become affiliated with the IWT through common interest such as Groundwork (International Volunteer Conservation Work Camps in National Parks), Bat Conservation Ireland (Umbrella Organisation for Bat Groups around Ireland) and Badgerwatch Ireland (Organisation involved in Badger Conservation, Welfare and Awareness).