 However, the use of bright exogenous dyes can provide substantially more remitted light than the autofluorescence approach.Several dyes have been studied for MUSE's application, including eosin, rhodamine, DAPI, Hoechst, acridine orange, propidium iodine, and proflavine.Eosin and rhodamine stain the cytoplasm and the extracellular matrix, making the bulk of the tissue visible.