 MUSE system mainly serves as a low-cost alternative to traditional histological analysis for cancer diagnostics with simpler and less time-consuming techniques.By integrating with microscopy and fresh tissue fluorescence staining into an automated optical system, the overall acquiring time needed for getting digital images with diagnostic values can be much shortened into scale of minutes comparing with conventional pathology, where general procedure can take from hours to days.The color-mapping techniques that correlated fluorescence staining to traditional H&E staining provide the same visual representation to pathologists based on existing knowledge system with no need of additional training on image recognition.