 Specimens are submerged in exogenous dye for 10 seconds and then briefly washed in water or phosphated buffered saline (PBS) .The resulting stained specimens generated bright enough signals for direct and interpretable visualization through microscope eyepiece.Previous work from MUSE includes the detection of endogenous fluorescent molecules in intact clinical and human tissues for functional and structural characterization, which is limited by the relatively dim autofluorescence found in tissue.