 SR 65 became part of State Route 66, which continued east from Dot to south of St. Paul along former State Route 70, in the 1940 renumbering, as Kentucky had taken over its continuation as Kentucky Route 66.In the meantime, the route from Cumberland Gap to Bristol had been through several changes in numbering.It was assigned U.S. Route 411 in 1926, renumbered as part of U.S. Route 58 in the early 1930s, and supplemented with U.S. Route 421 from the 1930s to the late 1940s.