 The overall process of termination is similar in bacteria, but in the latter 2 separate codon-recognizing release factors exist, RF1 and RF2.Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome.The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP.