 Puno was married to Luzviminda T. Delgado-Puno (1940–2006), a lawyer who had been the Clerk of Court of the Supreme Court from 1993 to 2005.Luz Puno (daughter of Dr. Gregorio and Anastacia Delgado) graduated cum laude (Bachelor of Laws) in 1961 at the University of the East, and passed the Bar examinations of that same year, when she was just 21 years old.Their children and their spouses are Reynato Jr. and Cheryl Mae H. Yap; Emmanuel and Rachelle Catherine Fabreo, and their only daughter Ruth Puno; their three grandchildren are Alessandra Isabelle, Laticia Raquelle and Elijah Rey Puno.