 In contrast, many life science fluorescence applications are indirect, consisting of a fluorescent dye increasing, decreasing, or shifting in wavelength emission upon binding to the molecule of interest.If good health physics controls are maintained in a laboratory where radionuclides are used, it is unlikely that the overall radiation dose received by workers will be of much significance.Nevertheless, the effects of low doses are mostly unknown so many regulations exist to avoid unnecessary risks, such as skin or internal exposure.