Atlantic, Suwannee River and Gulf Railway Atlantic, Suwannee River and Gulf Railroad Starke and Sampson City Railroad Brooksville and Inverness Railway Carolina, Atlantic and Western Railway Charleston Southern Railway Georgetown and Western Railroad Georgetown and Lane's Railroad Pee Dee Bridge Company North and South Carolina Railway Charleston Northern Railway North and South Carolina Railway South Carolina Western Railway South Carolina Western Extension Railway Carolina Central Railroad Carolina Central Railway Wilmington, Charlotte and Rutherford Railroad Wilmington and Charlotte Railroad Catawba Valley Railway Central Railway of Georgia (Lyons Branch only) Charlotte Harbor and Northern Railway Alafia, Manatee, and Gulf Coast Railroad Charlotte, Monroe and Columbia Railroad Chesterfield and Kershaw Railroad Chesterfield and Lancaster Railroad Columbus Railroad Brush Electric Light and Power Company Durham and Northern Railway Florida Central and Peninsular Railroad East Florida and Atlantic Railroad Orlando and Winter Park Railway Orlando and Lake Jesup Railway Fernandina and Amelia Beach Railway Florida Railway and Navigation Company Fernandina and Jacksonville Railroad Florida Central and Western Railroad Florida Central Railroad Florida, Atlantic and Gulf Central Railroad Jacksonville, Pensacola and Mobile Railroad Pensacola and Georgia Railroad Tallahassee Railroad Florida, Atlantic and Gulf Central Railroad (operated) Florida, Peninsula and Jacksonville Railroad Florida Transit and Peninsular Railroad Florida Transit Railroad Atlantic, Gulf and West India Transit Company Florida Railroad Peninsular Railroad Tropical Florida Railroad Jacksonville and Mobile Railroad Leesburg and Indian River Railroad Florida Northern Railroad Jacksonville Belt Railroad Santa Fe Canal Company Tavares, Orlando and Atlantic Railroad Florida, Peninsular and Gulf Railroad Arcadia, Gulf Coast and Lakeland Railroad Florida West Shore Railway United States & West Indies Railroad and Steamship Company Gainesville Midland Railroad (bought by Seaboard in 1959) Gainesville, Jefferson and Southern Railroad Walton Railroad Gainesville Midland Railway Gainesville and North Western Railroad Greene County Railroad Bostwick Railroad Georgia and Alabama Railway Abbeville and Waycross Railroad Albany and Florida Railroad Albany, Florida and Northern Railroad Columbus and Southern Railroad Columbus Southern Railway Columbus and Florida Railway Georgia and Alabama Terminal Company Savannah, Americus and Montgomery Railway Americus, Preston and Lumpkin Railroad Montgomery & West Point Railroad Georgia, Carolina and Northern Railway Chester, Greenwood and Abbeville Railroad Georgia, Florida and Alabama Railroad (gained control in 1928) Carrabelle, Tallahassee and Georgia Railroad Georgia, Florida and Alabama Railway Georgia Pine Railway Kissimmee River Railway Loganville and Lawrenceville Railroad Macon, Dublin and Savannah Railroad McRae Terminal Railway Oxford and Coast Line Railroad Line from Dickerson to Oxford, NC Palmetto