infant son James V, married Henry Stewart, 1st Lord Methven, a descendant of Albany. His brother, Sir James Stewart (c. 1513–1554), was made Captain of Doune Castle, and Sir James' son, also James (c. 1529–1590), was created Lord Doune in 1570. Lord Doune's son, another James (c. 1565–1592), married Elizabeth Stuart, 2nd Countess of Moray around 1580, becoming Earl of Moray himself. The castle thus came to be the seat of its keepers, the Earls of Moray, who owned it until the 20th century. Mary, Queen of Scots, (reigned 1542–1567) stayed at Doune on several occasions, occupying the suite of rooms above the kitchen. Doune was held by forces loyal to Mary during the brief civil war which followed her forced abdication in 1567, but the garrison surrendered to the Regent, Matthew Stewart, 4th Earl of Lennox, in 1570, after a three-day blockade. George Buchanan and Duncan Nairn, Deputy Sheriff of Stirling presided over the torture and interrogation of a messenger, John Moon, at Doune on 4 October 1570. Moon was carrying letters to Mary, Queen of Scots and Mary Seton. King James VI visited Doune on occasion, and in 1581 authorised £300 to be spent on repairs and improvements, the works being carried out by the master mason Michael Ewing under the supervision of Robert Drummond of Carnock, Master of Work to the Crown of Scotland. In 1593, a plot against James was discovered, and the King surprised the conspirators, who included the Earls of Montrose and Gowrie, at Doune Castle. Prison and garrison In 1607, the minister, John Munro of Tain, a dissenter against the religious plans of James VI, was imprisoned with a fellow minister at Doune, though he escaped with the contrivance of the then Constable of the Castle, who was subsequently imprisoned for aiding the dissenters. The Royalist James Graham, 1st Marquess of Montrose occupied Doune Castle in 1645, during the Wars of the Three Kingdoms. In 1654, during Glencairn's rising against the occupation of Scotland by Oliver Cromwell, a skirmish took place at Doune between Royalists under Sir Mungo Murray, and Cromwellian troops under Major Tobias Bridge. The castle was garrisoned by government troops during the Jacobite rising of 1689 of Bonnie Dundee, when repairs were ordered, and again during the rising of 1715. During the Jacobite rising of 1745, Doune Castle was occupied by Charles Edward Stuart, "Bonnie Prince Charlie", and his Jacobite