stress. Testing on the rats proved effective. In comparison to the rats that had not received the dye, the rats that were treated with the dye performed better on motion tests. It is unknown whether this treatment can be used effectively in humans. The animal experiments administered the dye within 15 minutes of injury, but to be effective in a real-life setting, where it may take time for a patient to reach the emergency room, the treatment needs to be effective even when administered up to two hours after injury. The only reported side effect was that the rats temporarily turned blue. Under the trade names ILM Blue and Brilliant Peel, brilliant blue G is used as a stain to assist surgeons in retinal surgery. In December 2019, brilliant blue G (under the trade name TissueBlue, DORC International, Netherlands) was approved for use in humans in the United States. Tissueblue was approved for medical use in Canada in January 2021. Application in forensic science The ability of the Coomassie dye to target amino acids with aromatic groups (phenylalanine, tyrosine, tryptophan) and basic side chains (lysine, arginine and histidine) allows the Bradford assay to be used for fingerprint analysis. The assay was successfully used to identify the biological sex of the fingerprint. Female samples were shown to have a higher absorbance than male samples when tested at similar wavelengths. This provides a simpler method for fingerprint analysis by reducing the number of amino acids needing to be analyzed from 23 to 6 and requires little to no assay preparation, in contrast to the ninhydrin chemical assay, which requires assay preparation such as heating and enzyme cascade. References Further reading External links Protein methods Triarylmethane dyes Staining dyes Phenol ethers Anilines Benzenesulfonates Benzenesulfonic acids Acid dyes