of China, west Korea and parts of mainland southeast Asia. The taxonomic position of certain populations, including some in China, Laos and east Korea, is unclear and require further study. It is possible that all these Chinese populations are part of O. sinensis, but the Laos specimens are relatively large, similar to O. latipes rather than the tiny O. sinensis. The east Korean population is part of a clade with O. sakaizumii and O. latipes. Based on morphology it is closer to O. sakaizumii than O. latipes, but it may be an undescribed species. O. latipes has been introduced to Hokkaido in northern Japan (where ricefish are not native). There are other reports of introductions around the world, but at least most of those in mainland Asia and Europe involve O. sinensis (Chinese rice fish). Origin of Southern and Northern Japanese populations Phylogenetic analysis shows that the southern Japanese population was derived from that of the northern Kyushu area and spread into Honshu. On the other hand, the northern population was derived from a population from the Tajima-Tango region and spread alongside the Sea of Japan coast. O. latipes is known to have nine sub-populations: East Japanese type, East Setouchi type, West Setouchi type, San'in type, Northern Kyushu type, Osumi Type, Ariake type, Satsuma type and Ryukyu type. These sub-populations have been mixed with each other due to artificial releasing and decreasing local genetic diversity. Use in science Oryzias latipes is a model organism and is extensively used in many areas of biological research, most notably in toxicology. Medaka have a short gestation period, and are reproductively prolific—characteristics that make them easy to rear in the laboratory. They can withstand cold and can be shipped easily. Nearly all aspects of the life cycle of medaka have been analyzed by researchers including sexual behavior, genetic inheritance of coloration, spawning habits, feeding, pathology, embryological development, ecology, etc. It has a relatively small genome (~800 mega base pairs, half the size of the genome of another popular model fish, the zebrafish) as well as a generation time of 7 weeks (rather than 9 weeks for zebrafish) and hardier growth in a broad temperature range (). Transgenic medaka are relatively easy to produce. They have been genetically modified to secrete various human hormones, express promoter sequences from other fish, and to make antimicrobial proteins and a protein that makes the medaka glow fluorescent green,