In enzymology, a prunasin beta-glucosidase () is an enzyme that catalyzes the chemical reaction (R)-prunasin + H2O D-glucose + mandelonitrile Thus, the two substrates of this enzyme are (R)-prunasin and H2O, whereas its two products are D-glucose and mandelonitrile. This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is prunasin beta-D-glucohydrolase. This enzyme is also called prunasin hydrolase. References EC 3.2.1 Enzymes of unknown structure