al. examined the 3D structure of P. aeruginosa malate synthase G. They found that it is a monomer composed of four domains and is highly conserved in other pathogens. They further utilized computational analysis to identify two binding pockets that may serve as drug targets. Brucella melitensis Brucella melitensis is a pathogenic bacterium that causes fever and inflammation of the epididymis in sheep and cattle and can be transmitted to humans through the consumption of unpasteurized milk. Malate synthase has been identified as a potential virulence factor in this bacterium. In 2016, Adi, et al. constructed a 3D crystallized structure of the protein to identify catalytic domains and investigate potential inhibitors. They identified five inhibitors with non-oral toxicity that served as drugs against the bacteria, suggesting possible treatment routes for brucellosis. Escherichia coli In Escherichia coli, the genes encoding the enzymes required for the glyoxylate cycle are expressed from the polycistronic ace operon. This operon contains genes coding for malate synthase (aceB), isocitrate lyase (aceA), and isocitrate dehydrogenase kinase/phosphatase (aceK). Structural Studies As of early 2018, several structures have been solved for malate synthases, including those with PDB accession codes 2GQ3, 1D8C, 3OYX, 3PUG, 5TAO, 5H8M, 2JQX, 1P7T, and 1Y8B. References EC 2.3.3 Enzymes of known structure