In enzymology, a 2-(acetamidomethylene)succinate hydrolase () is an enzyme that catalyzes the chemical reaction 2-(acetamidomethylene)succinate + 2 H2O acetate + succinate semialdehyde + NH3 + CO2 Thus, the two substrates of this enzyme are 2-(acetamidomethylene)succinate and H2O, whereas its 4 products are acetate, succinate semialdehyde, NH3, and CO2. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 2-(acetamidomethylene)succinate amidohydrolase (deaminating, decarboxylating). This enzyme is also called alpha-(N-acetylaminomethylene)succinic acid hydrolase. This enzyme participates in vitamin B6 metabolism. References EC 3.5.1 Enzymes of unknown structure