Molds such as Trichoderma, Exophiala, Stachybotrys, Aspergillus fumigatus, Aspergillus versicolor, Phialophora, Fusarium, Ulocladium and certain yeasts are used as indicators of indoor air quality. Metagenomic techniques allow for the sequencing of whole populations of microorganisms in a single operation. With metagenomic sequencing, it is possible to use the entire community of fungal organisms, or mycobiome in the soil or water of a given area as a biological indicator of anthropogenic activity, such as sewage overflow from an urban area or fertilizer and pesticide runoff from an agricultural one. Composition of fungal communities has been found to be a good indicator of environmental properties like pH, altitude and water temperature. Chauvet used this approach to take ecosystem-wide measurements of these variables using a network of monitoring stations at 27 streams in Southwestern France. Cudowski et al. sampled fungi in the water of the Augustow canal in eastern Poland. They took many standard measures of water quality -- temperature, oxygen saturation, pH, and dissolved nitrogen, organic carbon and sulfur levels. They identified species with microscopic methods and RFLP analysis. They found 38 fungal species, including 12 hyphomycetiae and 13 potential pathogens, belonging either to the dermatophytes or to relatives of C. albicans. Cudowski et al. found that they could determine whether a sample of water had been taken from the natural (lake-like) or artificial part of the canal. They also found that the three major groups of fungi that they found, hyphomycetes, dermatophytes and Candida relatives, could predict many of their water quality measurements, which formed two clusters in a redundancy analysis. Bouffand et al. used Arbuscular Mycorhizzal Fungi (AMF), an asexual clade of fungi that form symbiotic relationships with plant root systems, as indicators to assess soil function and biodiversity in many sites across Europe. They took soil samples in various climatic zones (atlantic, continental, mediterranean, alpine) and three land use regimes (arable, grassland, forestry), and sequenced the DNA of the fungi the soil contained. They found eight indicator species for soil pH: four that were only present when pH was less than 5, three for pH > 5 and one for pH > 7. They found eight indicators of land use: two for forests, five for farm- and grassland, and one for both. They also found one indicator fungus that was present when soil organic carbon was high, and another present when it was low. Indicator helminth eggs Helminth eggs