In enzymology, a cyanoalanine nitrilase () is an enzyme that catalyzes the chemical reaction 3-cyano-L-alanine + 2 H2O L-aspartate + NH3 Thus, the two substrates of this enzyme are 3-cyano-L-alanine and H2O, whereas its two products are L-aspartate and NH3. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in nitriles. The systematic name of this enzyme class is 3-cyano-L-alanine aminohydrolase. This enzyme is also called beta-cyanoalanine nitrilase. This enzyme participates in cyanoamino acid metabolism. References EC 3.5.5 Enzymes of unknown structure