including diffusion/mixing of ions in solution, the possibility of adsorption onto the surface, and the interaction between solvent dipole moments and the electrode. Gouy–Chapman Louis Georges Gouy in 1910 and David Leonard Chapman in 1913 both observed that capacitance was not a constant and that it depended on the applied potential and the ionic concentration. The "Gouy–Chapman model" made significant improvements by introducing a diffuse model of the DL. In this model, the charge distribution of ions as a function of distance from the metal surface allows Maxwell–Boltzmann statistics to be applied. Thus the electric potential decreases exponentially away from the surface of the fluid bulk. Gouy-Chapman layers may bear special relevance in bioelectrochemistry. The observation of long-distance inter-protein electron transfer through the aqueous solution has been attributed to a diffuse region between redox partner proteins (cytochromes c and c1) that is depleted of cations in comparison to the solution bulk, thereby leading to reduced screening, electric fields extending several nanometers, and currents decreasing quasi exponentially with the distance at rate ~1 nm−1. This region is termed "Gouy-Chapman conduit" and is strongly regulated by phosphorylation, which adds one negative charge to the protein surface that disrupts cationic depletion and prevents long-distance charge transport. Similar effects are observed at the redox active site of photosynthetic complexes. Stern The Gouy-Chapman model fails for highly charged DLs. In 1924, Otto Stern suggested combining the Helmholtz model with the Gouy-Chapman model: in Stern's model, some ions adhere to the electrode as suggested by Helmholtz, giving an internal Stern layer, while some form a Gouy-Chapman diffuse layer. The Stern layer accounts for ions' finite size and consequently an ion's closest approach to the electrode is on the order of the ionic radius. The Stern model has its own limitations, namely that it effectively treats ions as point charges, assumes all significant interactions in the diffuse layer are Coulombic, assumes dielectric permittivity to be constant throughout the double layer, and that fluid viscosity is constant plane. Grahame D. C. Grahame modified the Stern model in 1947. He proposed that some ionic or uncharged species can penetrate the Stern layer, although the closest approach to the electrode is normally occupied by solvent molecules. This could occur if ions lose their solvation shell as they approach the electrode. He called ions in direct contact with the electrode "specifically adsorbed ions". This model proposed the existence of three regions.