Physalaemus santafecinus is a species of frog in the family Leptodactylidae. It is found in Argentina and possibly Paraguay. Its natural habitats are temperate grassland, intermittent freshwater lakes, intermittent freshwater marshes, and pastureland. It is threatened by habitat loss. References santafecinus Amphibians of Argentina Taxonomy articles created by Polbot Amphibians described in 1965