fundamental frequency was believed to be 500 Hertz while the dominant frequencies were considered to be around 800 Hertz.. Additionally, males that tend to be more vocal, tend to travel greater distances in the environment. However, some males have been reported to stay in place while calling out to other frogs. In the context of silent communication, it is relevant to also consider that the frogs, specifically the males, conduct a leg-waving behavior to demonstrate their strength to foes. This can be done to avoid physical fighting. Breeding site attendance Most female only attend once in breeding, but for those who attended more than once, the nesting interval averaged 27.6 days. Males showed high pond loyalty; a few participate in two ponds while always preferring one over the other. There are three attendance patterns for men. Stays for multiple nights, sporadic attendance, and only once. Most frogs appearing on multiple nights. However, there is also no evidence that a particular model is the best choice for reproductive success. Development and life cycle Physical development The first paper to explain the development of toe pads in Phyllomedusa trinitatis used light and scanning electron microscopy to show that the adult toe pad has some mucosal pores in a sea of "hexagonal shaped cells". The study also found that the frog has pads that are flat and lack “lateral grooves” on the front of each individual digit. This distinguishes the Trinidad frog from other tree frogs or hylids that have differing features, such as convex pads. Additionally, P. trinitatis is described to have about 12 cell layers—including columnar and cuboidal cells—on its toe pad. Using Gosner's (1960) staging table for frog development, they showed the stage by stage changes that take place in frog toe pads. For the purposes of this page, when a stage is mentioned it will be in reference to Gosner's staging paradigm rather than Kenny's staging (1968) for example. They noted that in earlier stages of development, the forelimb appears to develop at a faster rate than the hindlimb based on how separated the digits were. By stage 38, all digits were layered with an epithelium of simple squamous cells. At stage 39, the long toe had a notably expanded distal end and the toe pad had widened. At stage 40, the circumferal groove became very apparent in the toe pads and was fully developed by stage 46, the