lepidoptera (11.02%). Other prey found in the stomach contents include: blattaria, hemiptera, acari, hymenoptera, orthoptera. An average of 1.5 items were found per frog stomach. There was no significant correlation between frog body mass or snout-vent length and ingested volume of prey. Stomach content analysis of D. branneri exhibited significant plant content (13.39% of total volume). It is unclear whether this ingestion is intentional or an accidental consequence of arthropod hunting. D. branneri are the first species in which stomach flushing was used as a mechanism for studying the diet of frogs smaller than 14.4mm snout vent length (SVL). Whereas, the stomach content analysis of its closely related species, D. minutus, sampled in Serra Norte, Carajás, Brazil presented a slightly different prey composition. In D. minutus, Araneae was found to be the dominant prey-type. Larger frog species P. rohdei and P. burmeisteri tend to prey on larger invertebrates compared to D. branneri, likely related to a larger mouth size. Mating Breeding season The D. branneri breeding season lasts from May to September, with the highest reproductive activity concentrated during the rainy season. During the breeding season, D. branneri aggregate and lay eggs on the water surface. D. branneri are known to have a uniquely long breeding season compared to other frogs. Unlike most frog species that can only utilize temporary ponds for breeding, D. branneri is able to breed in both temporary and permanent pools. This allows D. branneri to inhabit a comparatively wide variety of habitats including savanna, shrubland, lowland grassland, freshwater marshes, and human-impacted areas such as pastureland. Male/male interactions D. branneri males are more willing to escalate aggressive behavior compared to other frog species, who prefer to avoid combat and associated injury risk. Preliminary confrontational behaviors typically include chasing and calls of aggression. These behaviors can then escalate into more aggressive behaviors such as kicking, pushing, and other physical attacks, for example use of anterior limbs to force the opponent into a non-dominant position. Fights are normally avoided as it can be costly due to injuries. Analysis of vocalizations has demonstrated distinct differences between fighting calls and advertisement calls. Compared to advertisement calls, fighting calls exhibit lower dominant frequency (4866.50 vs. 6350 Hz), longer duration (0.2492 vs. 0.03 seconds), and higher number of pulses per call (62.3 vs. 4). The reasons for fighting calls have not been identified as calls may be costly because of extra energy