end of metamorphosis. Notably, researchers have claimed that unlike other species of frogs, P. trinitatis has no evidence of having hatching gland cells during its development. Looking at Gosner stages 18 to 23, the scientists did not see hatching gland cells on the heads of the frogs. These findings suggest that P. trinitatis might have a different hatching mechanism distinct from other frogs of other species. Other studies, however, have shown conflicting results. Embryos farther along in their development were shown to have hatching gland cells on the laterodorsal surface of the head. Life cycle Concerning the nesting behaviors of P. trinitatis, it appears to exhibit similar behavior to other Phyllomedusa frogs. Once an egg clutch is produced, it can sometimes be found as high as trees or as low as grass when better leaves for nesting cannot be found. It is known that P. trinitatis hatches on leaves that are nearby water. As a result, the tadpoles fall into the water once fully mature. Usually, when the egg clutch is released, it is surrounded by a leaf to separate it from the environment and offer protection. This configuration may sometimes shield the clutch from rainfall. This makes sense considering that incubation in water kills Phyllomedusa eggs, a unique phenomenon for an amphibian species. The mechanism by which such embryos paradoxically survive with potential issues of hypoxia are yet to be discovered. The eggs are later covered with jelly capsules or plugs produced by the mother. The jelly plugs have been reported to be made of 96 to 97% water and 2 to 3% dry matter. The jelly capsules possess a dense core surrounded by a matrix. It has been shown that the capsules are positive for alcian blue while the matrix is positive for periodic acid-Schiffs, indicating a composition of different mucopolysaccharides. It also appears that the jelly plug and capsule prevent water absorption during rainfall. Researchers reported that P. trinitatis has no evidence of leaf preference when deciding on where to have their nest. The same can be said for the number of leaves they used. After observing 17 nests, they found that 35% of the frogs used 2 leaves, 41% used 3, 12% used 4, and 12% used more than 4. Hatching success has also been tested on dead or healthy leaves and no significant difference was observed between control and experimental groups. Of note, as one