frog. The advertisement call of T. midas consists of a single pulsed note with 3 pulses in very short emission. Series duration varies from 0.7 s to 61.8s. However, advertisement calls may vary due to geographical location. For example, calls from Ecuador presented 4 pulses/call, while in French Guiana and Brazil, they are composed of 3 pulses/call. The duration of calls also varies among geographical locations. Some populations in French Guyana have longer pulse duration than the population in Brazil. Though there is little data regarding reproductive ecology, observations have been recorded of a female hopping from leaf to leaf with the male on the back and maintaining its amplexus in the nuchal and axillary amplexus position. One research on T. midas in the Colombian Amazon suggests a pattern in the species' oviposition, the location at which female frogs repel their egg from the oviduct to the external environment. Female usually lay their eggs on rocks or plants above water so that when the eggs hatches into tadpoles, the tadpoles can directly fall into waterbodies. Parental care Oviposition T. midas has a tendency to choose a plant genus called Selaginella as the oviposition site. Researchers have hypothesized that because the plants have antibacterial and antifungal properties, they may protect the eggs from common fungus infections such as Saprolegnia. Additionally, these moth-like plants have scaled leaves which make their surface rougher. The uneven edges of the leaves would then increase tension between the clutches and the leaves, thus preventing displacement of the clutches due to wind or rain. Finally, there is no general pattern for the height at which T. midas lay their eggs. Egg Protection/Guarding After fertilization has occurred, the male would leave the oviposition site while the female remains to cover the eggs. The egg-laying process only lasts for a few seconds, but the female would continue to keep the eggs under its ventral or belly area for around an hour. Based on observations where the eggs seem more moisturized, one hypothesis the researchers have made is that this attendance action would keep the eggs more hydrated and protect them from future predation or danger. This reproductive behavior is relatively common among other glass frog species. An experiment has proven that female attendance of eggs keeps eggs moisturized in a specie called L. Tayrona, which is in the same family, Centrolenidae, as T. midas. Growth and development T. midas