regulating their core temperature, yet maintaining enough heat to kill off the chytrid fungus. The antimicrobial skin peptides in the skin of lemur leaf frogs have shown to be a strong preventative measure for chytrid fungus, in particular Batrachochytrium dendrobatidis. In 2004 a sample of peptides from nine different adult amphibians in Omar Torrijos National Park, Panama were taken to test for susceptibility to chytridiomycosis. Of the 9 species tested the lemur leaf frog ranked third in immunologic resistance with a mean of 15% in inhibition of pathogen growth and peptide renewal. The strongest (Xenopus Laevis) had a mean of 65% more than four times that of the lemur leaf frog. Although the lemur leaf frog shows some resilience to chytridiomycosis it is not completely immune. In a different study following that, it was predicted that this species could survive chytridiomycosis because they contained dermaseptin-L1 and phylloseptin-L1 peptides which contained resistance to gram-negative and gram-positive bacteria. While there has been survivors, the appearance of chytridiomycosis still caused population decline despite their antimicrobial skin peptides. This shows that in-vitro studies may not represent what actually happens in living organisms. By taking skin secretions of lemur leaf frogs, scientists tested to conclude that the skin peptides contained phylloseptin-L1 and dermaseptinL1 with cytolytic activities in order to combat bacteria and fungus. For these reasons, at Omar Torrijos National Park, it was predicated that the species would be able to survive the appearance of chytridiomycosis. While there have been survivors, a large population decrease was caused from chytridiomycosis despite the anti-fungi properties of their skin peptides. This shows that the results that are taken in a study may be different than what happens in real life. Conservation In Costa Rica, lemur leaf frogs are currently only found in two remaining locations. Genetic testing of the mitochondrial DNA of the lemur leaf frog populations show that those frogs are distinct from the Panamanian frogs. This highlighted the need for the separate conservation of the Costa Rican populations. The first in situ conservation effort for the lemur leaf frog was started in 2003. The in-situ aspect of the project was carried out by the Costa Rican Amphibian Research Center, consisting of introducing tadpoles to artificial ponds every year, gradually increasing the wild population of the frogs inside of the reserve. The project has been so successful that the frogs are spreading out from the center. It