calcium readily reacts to form calcium carbonate instead of apatite minerals. With the free calcium ions removed from solution, phosphate ions are no longer precipitated from solution. This is specifically seen in lakes with no inflow, since no new calcium is introduced into the water body. After all of the calcium is sequestered into calcium carbonate (calcite), phosphate concentrations are able to increase to levels necessary for facilitating biomolecule creation. Though carbonate-rich lakes have alkaline chemistry in modern times, models suggest that carbonate lakes had a pH low enough for prebiotic synthesis when placed in the acidifying context of Earth’s early carbon dioxide rich atmosphere. Rainwater rich in carbonic acid weathered the rock on the surface of the Earth at rates far greater than today. With high phosphate influx, no phosphate precipitation, and no microbial usage of phosphate at this time, models show phosphate reached concentrations approximately 100 times greater than they are today. Modeled pH and phosphate levels of early Earth carbonate-rich lakes nearly match the conditions used in current laboratory experiments on the origin of life. Similar to the process predicted by geothermal hot spring hypotheses, changing lake levels and wave action deposited phosphorus-rich brine onto dry shore and marginal pools. This drying of the solution promotes polymerization reactions and removes enough water to promote phosphorylation, a process integral to biological energy storage and transfer. When washed away by further precipitation and wave action, researchers conclude these newly formed biomolecules may have washed back into the lake - allowing the first prebiotic syntheses on Earth to occur. Environmental and evolutionary impact of microbial mats Microbial mats are multi-layered, multi-species colonies of bacteria and other organisms that are generally only a few millimeters thick, but still contain a wide range of chemical environments, each of which favors a different set of microorganisms. To some extent each mat forms its own food chain, as the by-products of each group of microorganisms generally serve as "food" for adjacent groups. Stromatolites are stubby pillars built as microorganisms in mats slowly migrate upwards to avoid being smothered by sediment deposited on them by water. There has been vigorous debate about the validity of alleged stromatolite fossils from before 3 Ga, with critics arguing that they could have been formed by non-biological processes. In 2006, another find of stromatolites was reported from the same part of Australia, in rocks dated to 3.5 Ga. In