discovered homologs of 6S are two Bacillus subtilis RNAs and cyanobacterial RNAs. Two 6S RNA, 6S-1 and 2 along with their encoding genes bsrA and B present at various positions of a genome. In stationary phase deletion of 6S-1 in B. subtilis results in inhibition of its growth. The absence of 6S-2 RNA, on the other hand, does not appear to influence growth and sporulation in the stationary phase. 6S RNA conserved feature shows that it binds to the RNA polymerase by replicating the structure of DNA template. Promoter-dependent transcriptional regulation is mediated by 6S RNA as some of the promoters may be down-regulated and some are insensitive in the presence of 6S RNA. Gene expression studies revealed that 6S RNA is integrated in different global pathways e.g., it regulates various factors that influence transcription like Crp, FNR etc. and translation mechanism. Scientist discovered that 6S RNA binds with the active site of RNA polymerase and can serve as a template for RNA synthesis required for the RNA synthesis. It down-regulates transcription from 3´-5´fold at various promoters but doesn't inhibit transcription during late stationary phase. In a nutrient-deficient environment, 6S RNA control transcription leads to altered cell survival, possibly through redirecting resource consumption. Through SDS-PAGE analysis 6S RNA was identified present in E. coli and cover almost 25% of the total ribosomal number. 1000-1500 molecules were estimated to be present in E. coli genome. Although 6S RNA does not appear to be associated with ribosomes, it does appear to be complexed with several proteins and migrates at around 11S. 6S RNA is a regulator of RNA polymerase and abundantly present in bacteria. Studies has shown that the 6S RNA forms a complex with RNA polymerase to initiate transcription. Lack of 6S RNA in cells result in altered phenotypes. A unique feature of 6S RNA is that it acts like a template for RNA synthesis and the length and abundance of RNAs vary according to cell physiology. pRNA synthesis is critical as it releases RNA polymerase that allows the inhibition to be reversed. Structural and functional analyses showed the interactions between RNA polymerase and E. coli 6S RNA. The functional variety of 6S RNAs was discovered by genome-wide transcriptome studies. Numerous recent investigations have suggested that 6S RNA serves as a guardian, regulating the efficient utilisation of cellular resources under restricted conditions and stress. By interacting with the sigma 70-dependent RNA