possible to introduce functional groups into imprinted cavities through non-covalent interactions, thus leading to non-covalent imprinting. Many approaches regarding molecular imprinting have since been extended to different purposes. Type of Molecular Imprinting Covalent In covalent imprinting, the template molecule is covalently bonded to the functional monomers that are then polymerized together. After polymerization, the polymer matrix is cleaved from the template molecule, leaving a cavity shaped as the template. Upon rebinding with the original molecule, the binding sites will interact with the target molecule, reestablishing the covalent bonds. During this reestablishment, kinetics associated with bond binding and bond breakage are obtained back. The imprinted molecule is then released from the template, in which it would then rebind with the target molecule, forming the same covalent bonds that were formed before polymerization. Advantages through utilizing this approach include the functional group being solely associated with the binding sites, avoiding any non-specific binding. The imprinted molecule also displays a homogenous distribution of binding sites, increasing the stability of the template-polymer complex. However, there are a few number of compounds that can be used to imprint with template molecules via covalent bonding, such as alcohols, aldehydes and ketones, all of which have high formation kinetics. In some cases, the rebinding of the polymer matrix with the template can be very slow, making this approach time inefficient for applications that require fast kinetics, such as chromatography. Non-covalent With non-covalent imprinting, interaction forces between template molecule and functional monomer are the same as the interaction forces between the polymer matrix and analyte. The forces involved in this procedure can include hydrogen bonds, dipole dipole interactions, and induced dipole forces. This method is the most widely used approach to create MIPs due to easy preparation and the wide variety of functional monomers that can be bound to the template molecule. Among the functional groups, methacrylic acid is the most commonly used compound due to its ability to interact with other functional groups. Another way to alternate the non-covalent interaction between the template molecule and polymer is through the technique ‘bite and switch’ developed by Professor Sergey A. Piletsky and Sreenath Subrahmanyam. In this process, functional groups first non-covalently bond with the binding site, but during the rebinding step, the polymer matrix forms irreversible covalent bonds with the target molecule. Ionic/Metallic Ionic imprinting, which involves metal ions, serves as an approach to enhance template molecule and functional