| Robert W. Upton (R) | August 14, 1953 |- | Ohio(3) | nowrap | Robert A. Taft (R) | Died July 31, 1953.Successor appointed November 10, 1953. | nowrap | Thomas A. Burke (D) | November 10, 1953 |- | Nebraska(2) | nowrap | Dwight Griswold (R) | Died April 12, 1954.Successor appointed April 16, 1954. | nowrap | Eva Bowring (R) | April 16, 1954 |- | North Carolina(3) | nowrap | Clyde R. Hoey (D) | Died May 12, 1954.Successor appointed May 12, 1954 and then elected November 2, 1954. | nowrap | Sam Ervin (D) | June 5, 1954 |- | Wyoming(2) | nowrap | Lester C. Hunt (D) | Died June 19, 1954.Successor appointed June 24, 1954. | nowrap | Edward D. Crippa (R) | June 24, 1954 |- | Nebraska(1) | nowrap | Hugh A. Butler (R) | Died July 1, 1954.Successor appointed July 3, 1954. | nowrap | Samuel W. Reynolds (R) | July 3, 1954 |- | South Carolina(2) | nowrap | Burnet R. Maybank (D) | Died September 1, 1954.Successor appointed September 6, 1954. | nowrap | Charles E. Daniel (D) | September 6, 1954 |- | Nevada(3) | nowrap | Pat McCarran (D) | Died September 28, 1954.Successor appointed October 1, 1954. | nowrap | Ernest S. Brown (R) | October 1, 1954 |- | Nebraska(1) | nowrap | Samuel W. Reynolds (R) | Did not run in the special election to fill seat.Successor elected November 2, 1954. | nowrap | Roman Hruska (R) | November 8, 1954 |- | Nebraska(2) | nowrap | Eva Bowring (R) | Did not run in the special election to fill seat.Successor elected November 2, 1954. | nowrap | Hazel Abel (R) | November 8, 1954 |- | New Hampshire(3) | nowrap | Robert W. Upton (R) | Lost special election to fill seat.Successor elected November 2, 1954. | nowrap | Norris Cotton (R) | November 8, 1954 |- | North Carolina(2) | nowrap | Alton Lennon (D) | Lost special election to fill seat.Successor elected November 2, 1954. | nowrap | W. Kerr Scott (D) | November 29, 1954 |- | Wyoming(2) | nowrap | Edward D. Crippa (R) | Did not run in the special election to fill seat.Successor elected November 2, 1954. | nowrap | Joseph C. O'Mahoney (D) | November 29, 1954 |- | Nevada(3) | nowrap | Ernest S.