(D) | style="font-size:80%" | Retired December 21, 1860 | Vacant | Not filled this term |- | | | John D. Ashmore (D) | style="font-size:80%" | Retired December 21, 1860 | Vacant | Not filled this term |- | | | William W. Boyce (D) | style="font-size:80%" | Retired December 21, 1860 | Vacant | Not filled this term |- | | | Israel Washburn Jr. (R) | style="font-size:80%" | Resigned January 1, 1861, after being elected Governor of Maine | | Stephen Coburn (R) | Seated January 2, 1861 |- | | | Reuben Davis (D) | style="font-size:80%" | Withdrew January 12, 1861 | Vacant | Not filled this term |- | | | William Barksdale (D) | style="font-size:80%" | Withdrew January 12, 1861 | Vacant | Not filled this term |- | | | Otho R. Singleton (D) | style="font-size:80%" | Withdrew January 12, 1861 | Vacant | Not filled this term |- | | | John J. McRae (D) | style="font-size:80%" | Withdrew January 12, 1861 | Vacant | Not filled this term |- | | | James A. Stallworth (D) | style="font-size:80%" | Withdrew January 21, 1861 | Vacant | Not filled this term |- | | | James L. Pugh (D) | style="font-size:80%" | Withdrew January 21, 1861 | Vacant | Not filled this term |- | | | David Clopton (D) | style="font-size:80%" | Withdrew January 21, 1861 | Vacant | Not filled this term |- | | | Sydenham Moore (D) | style="font-size:80%" | Withdrew January 21, 1861 | Vacant | Not filled this term |- | | | George S. Houston (D) | style="font-size:80%" | Withdrew January 21, 1861 | Vacant | Not filled this term |- | | | Jabez L. M. Curry (D) | style="font-size:80%" | Withdrew January 21, 1861 | Vacant | Not filled this term |- | | | George S. Hawkins (D) | style="font-size:80%" | Withdrew January 21, 1861 | Vacant | Not filled this term |- | | | Peter E. Love (D) | style="font-size:80%" | Retired January 23, 1861 | Vacant | Not filled this term |- | | | Martin J. Crawford (D) | style="font-size:80%" | Withdrew January 23, 1861 | Vacant | Not filled this term |- | | | Thomas Hardeman Jr. (O) | style="font-size:80%" | Withdrew January 23, 1861 | Vacant | Not filled this term |- | | | Lucius