The northeastern corner of a subdivision and a high voltage transmission line meet Highbury Avenue at the intersection of Elgin County Road52 (Ron McNeil Line), after which the road enters the rural township of Central Elgin. Through Central Elgin, the road is designated as Elgin County Road 30. Dipping into the Kettle Creek valley, Highbury Avenue passes the trailhead of the Dan Patterson Conservation Area at Mapleton Line. It crosses Salt Creek and Kettle Creek within the valley before returning to an agricultural setting at Elgin County Road48 (Ferguson Line). Approaching the Elgin County boundary, the road swerves northwest as it transitions from the Lake Erie-oriented survey of Elgin County to the Thames River-oriented survey of former Westminster Township. Highbury Avenue crosses from Central Elgin into the city of London at the intersection of Webber Bourne and Thompson Line, although the surroundings remain agricultural. It travels between farm fields for the next , with the LEED Certified London Southeast Pumping Station and Reservoir near the ghost village of Hubrey breaking the monotony. Leaving the farmland to cross two branches of Dingman Creek, Highbury Avenue widens to four lanes, and enters urban London at Wilton Grove Road, where it divides the Wilton Grove Industrial Park. Urban London Proceeding north, Highbury Avenue curves northwest as it crosses Highway401 at Exit189. North of that interchange, the opposing carriageways separate as Highbury Avenue transitions into a controlled-access highway. It curves back northward parallel to and east of high-voltage transmission lines before encountering an interchange with Bradley Avenue. It continues, sandwiched between the established neighbourhood of Pond Mills and the developing neighbourhood of Summerside, to an interchange with Commissioner's Road. It then begins to descend into the Thames River valley as it travels west of the Meadowlily Nature Preserve. Crossing the Thames River and the Thames Valley Parkway recreational trail, the freeway portion of Highbury Avenue ends at Power Street, alongside the Highbury transformer station. It transitions back to an arterial road as it approaches Hamilton Road, with detached residences lining the route between Hamilton Road and Trafalgar Street. After crossing over the Canadian National Railway (CN) Dundas subdivision and passing the London Transit main office, it continues between residences and crosses the CN Guelph subdivision at grade. Between Dundas Street (former Highway 2) and Oxford Street, Highbury Avenue crosses over the Canadian Pacific Railway (CP) Galt subdivision and travels along the western side of