state of 129Xe are existing experimentally doable temperature and pressure ranges. Xenon possesses highly polarizable electron cloud surrounding the nucleus. Therefore, easily prone to be soluble with lipid or organic compounds especially in vivo environment in biological respect. (table-2) Xenon does not alter the structurally or chemically (similarly other noble gases) when interacting with other molecules. According to the scientist Ostwald, solubility is defined as the partition coefficient of the gas absorbed to the volume of the absorbing liquid. The solubility of Xenon, SXe(g) = V absorbed amount of Xe(g) /V absorbing liquid at standard temperature and pressure (STP). Solubility of Xenon in water medium 11% means at 25 °C 11 mL Xenon gas could be absorbed by 100 mL of water. Xenon atomic size is large and outer shell electrons are far from the nuclei, outermost electron is highly prone to be polarized specially lipid environment. Table 2 shows Xenon solubility in water medium at 37 °C is 8% but fat tissue in vivo environment the solubility value is 130%. Solubility leads the Xenon using in biological system as a contrasting agent. Solvent effect of xenon is very large for of 129Xenon by the fact of solubility (table 2). Chemical shift value range for Xenon is more than 7500 ppm. However solvent effect is limited range for 1H & 13C (MRI active nuclei) because of low chemical shift value range for 1H is 20 ppm and for 13C is 300 ppm. Therefore, using the 129Xe is preferred. Figure-9 below, In NMR experimental data, there are different chemical shift values for different tissues in in vivo environment. All peaks are positioned through such a big range of chemical shift values for 129Xe is viable. Because 129Xe has long range up-to 1700ppm chemical shift value range in NMR data. Other important spectral information includes: Figure 9. NMR data for Xe-129 biosensor in in vivo biological system. Naturally 129Xe NMR peak has been counted as reference at 0.0ppm. When 129Xe incorporated and bind with the Cryptophane-A molecule, then the chemical shift value in NMR acquisition shifted to around 70ppm. If hyperpolarized 129Xe gas is dissolved into the brain, then five NMR spectral peaks can be observed. Among them top sharp peak at 194.7ppm. In addition, at 189 ppm peak come out from the non-brain tissues. Another two peaks are still unknown at 191.6 ppm and 197.8 ppm. At 209.5 ppm smaller but