maintained a Mesolithic lifestyle for about 1500 years after farming arrived in Central Europe. The emergence of the Neolithic British Isles through maritime colonization by Michelsberg-related groups occurred almost at the same time as the expansion of the Funnelbeaker culture into Scandinavia, suggesting that these events may be connected. Although they were largely of Early European Farmer (EEF) descent, people of the Funnelbeaker culture had a relatively high amount of hunter-gatherer admixture, particularly in Scandinavia, suggesting that hunter-gatherer populations were partially incorporated into it during its expansion into this region. People of the Funnelbeaker culture often had between 30% and 50% hunter-gatherer ancestry depending on the region. During later phases of the Neolithic, the Funnelbeaker culture re-expanded out of Scandinavia southwards into Central Europe, establishing several regional varieties. This expansion appears to have been accompanied by significant human migration. The southward expansion of the Funnelbeaker culture was accompanied by a substantial increase in hunter-gatherer lineages in Central Europe. The Funnelbeaker communities in Central Europe which emerged were probably quite genetically and ethnically mixed, and archaeological evidence suggests that they were relatively violent. From the middle of the 4th millennium BC, the Funnelbeaker culture was gradually replaced by the Globular Amphora culture on its southeastern fringes, and began to decline in Scandinavia. In the early 3rd millennium BC, the Corded Ware culture appeared in Northern Europe. Its peoples were of marked steppe-related ancestry and traced their origins in cultures further east. This period is distinguished by the construction of numerous defensive palisades in Funnelbeaker territory, which may be a sign of violent conflict between the Funnelbeakers, Corded Ware, and Pitted Ware. By 2650 BC, the Funnelbeaker culture had been replaced by the Corded Ware culture. Genetic studies suggest that Funnelbeaker women were incorporated into the Corded Ware culture through intermixing with incoming Corded Ware males, and that people of the Corded Ware culture continued to use Funnelbeaker megaliths as burial grounds. Subsequent cultures of Late Neolithic, Bronze Age, and Iron Age Central Europe display strong maternal genetic affinity with the Funnelbeaker culture. Distribution The TRB ranges from the Elbe catchment in Germany and Bohemia with a western extension into the Netherlands, to southern Scandinavia (Denmark up to Uppland in Sweden and the Oslofjord in Norway) in the north, and to the Vistula catchment in Poland and the area between Dnister and Western Bug headwaters in Ukraine in the east. Variants of