broad peak has been found in NMR data when 129Xe was dissolved in the blood stream. Hyperpolarized 129Xe is very sensitive detector of biomarker (form of cancer in living system). The nuclear spin polarization of 129Xe or in generally for noble gases we can increase up to fivefold via SEOP technique. Using SEOP hyperpolarization technique, we can get images of uptake of xenon in the human brain tissue. (Figure-10) 129Xe(g) shows satisfactory enhancement in polarization during SEOP compared to the thermal enhancement in polarization. This is demonstrated by the experimental data values when NMR spectra are acquired at different magnetic field strengths. A couple of important points from experimental data are: The 129Xe polarization has increased about 144,000-fold in SEOP tech. over thermally enhanced for 1H polarization in NMR experiment. Both experiments that showed this have been done in identical conditions and using same radio frequency during NMR experiment. A similar value of 140,000-fold signal enhancement for 129Xe hyperpolarization in SEOP compare to the reference thermally enhanced 13C NMR signal is also seen in experimental NMR data. Both data have been collected in identical Larmor frequency and other experimental conditions and same radio frequency during NMR data collection. (Figure 11) Longitudinal spin relaxation time (T1) is very sensitive with an increase of magnetic field and hence enhance the NMR signals is noticeable in SEOP in case of 129Xe. As T1 is higher for blue marking conditioning NMR experiment shows more enhanced peak compare to other. For hyperpolarized 129Xe in tedlar bags, the T1 is 38±12 minutes when data collected in presence of 1.5 mT magnetic field. However, satisfactory increment in T1delay time (354±24 minutes) when data was collected in presence of 3000 mT magnetic field. Use of Rb vs. Cs in SEOP NMR Experiments In general, we can use the either 87Rb or 133Cs alkali metal atoms with inert nitrogen gas. However, we are using 133Cs atoms with nitrogen to make the spin exchange with 129Xe for number of advantages: 133Cs has natural perfect abundance while rubidium has two (85Rb and 87Rb) isotopes. Abstraction of one isotope separately from these two (85Rb and 87Rb) is difficult compare to collect the 133Cs isotope. Abstraction of 133Cs is convenient. Optical pumping cell normally is operated at lower temperature to avoid chemically breakdown issue. SEOP is using 133Cs at low temperature and hence it has fewer chemical corrosion with SEOP cell wall glass.