U.S. Route 301 (US 301) is a spur of U.S. Route 1 running through the South Atlantic States. It runs from Biddles Corner, Delaware at Delaware Route 1 to Sarasota, Florida at U.S. Route 41. It passes through the states of Delaware, Maryland, Virginia, North Carolina, South Carolina, Georgia, and Florida. It goes through the cities of Middletown, Delaware; Annapolis, Maryland; Richmond and Petersburg, Virginia; Rocky Mount, Wilson, and Fayetteville, North Carolina; Florence, South Carolina; Statesboro and Jesup, Georgia; and Ocala, Zephyrhills, and Sarasota, Florida. US 301 parallels Interstate 95 for much of its routing through The Carolinas and Virginia including short concurrencies in Santee, South Carolina and Lumberton, North Carolina. It has a number of other concurrencies along its route. Originally US 301 was much shorter. Its former northern terminus was in Baltimore, Maryland. It followed the alignment of the current Maryland Route 3, portions of the Baltimore Beltway, and Maryland Route 648. US 301 ended in Southwestern Baltimore on Monroe Street at the intersection with US 1. (Later, MD 3 was supplanted north of Millersville by Interstate 97.) It was later extended into the state of Delaware, along a different alignment that bypassed Baltimore altogether. Until 2019, the northern terminus was at US 40 in Glasgow, Delaware; the state of Delaware built a new tolled freeway to carry US 301, and it terminates southeast of the former terminus, at Delaware Route 1 just south of the Chesapeake & Delaware Canal. The original southern end was in Summerton, South Carolina, and it has been extended south many times to US 41 in Sarasota, Florida. Route description |- |FL |266 |428 |- |GA |169 |272 |- |SC |190.3 |306.3 |- |NC |194 |312.2 |- |VA |145 |233 |- |MD |123.30 |198.43 |- |DE |11.9 |24.03 |- |Total |1099 |1,769 |} Florida Concurrencies in Florida include US 1/23 between Folkston (Georgia) and Callahan, US 441 between Sparr and Belleview, US 27 between Ocala and Belleview, US 98 between Moss Town and Clinton Heights (near Dade City), and US 41 between Palmetto and South Bradenton. The road is also shared by the DeSoto Trail south of Bushnell. Georgia In Georgia, US 301 is signed concurrently with various state routes. It uses SR 4/SR 15 from Florida to Folkston, SR 23 from Folkston to Glennville, and SR 73 from Glennville to South Carolina. Large portions of US 301 in Georgia are concurrent with