Biophysical Journal. Vol. 104, nº 2; 279–280. 2014, D.M. Gilkes, L. Xiang, S.J. Lee, M.E. Hubbi, P. Chaturvedi, D. Wirtz, and G.L. Semenza, Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells, in: PNAS. Vol. 111; E384-E393. 2014, P.H. Wu, A. Giri, S.X. Sun, D. Wirtz, Three-dimensional cell migration does not follow a random walk, in: PNAS. Vol. 111, nº 11; 3949–3954. 2015, P.-H. Wu, A. Giri, and D. Wirtz, Statistical analysis of cell migration in 3D using the anisotropic persistent random walk model, in: Nature Protocols. Vol. 10; 517–527. Cell and nuclear mechanics Wirtz developed novel tools and concepts to study the role of nucleus and nuclear connections to the cytoskeleton and key cell functions, including 2D and 3D cell migration and adhesion, mechanosensation, and mechanotransduction. In particular, he identified LINC complexes and the so-called perinuclear actin cap as key mediators of physical signaling between the cytoplasm and the nuclear interior. More recently, he discovered that the nuclear lamina of adherent cells is polarized through the actin cap, which in turn polarizes hyper-acetylated forms of histones in the nucleus. Supporting Publications: 2009, S.B. Khatau, C.M. Hale, P.J. Stewart-Hutchison, M.S. Patel, C.L. Stewart, P.C. Searson, D. Hodzic, and D. Wirtz, A perinuclear actin cap regulates nuclear shape, in: PNAS. Vol. 107; 19017–19022. 2013, D.H. Kim, A.B. Chambliss, and D. Wirtz, The multi-faceted role of the actin cap in cellular mechanosensation and mechanotransduction, in: Soft Matter. Vol. 9; 5516–5523. 2013, A.B. Chambliss, S.B. Khatau, P.-H. Wu, N. Erdenberger, D.K. Robinson, D. Hodzic, G.D. Longmore, and D. Wirtz, The LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction, in: Scientific Reports. Vol. 3; 1087–1095. 2013, S.B. Khatau, R.J. Bloom, S. Bajpai, D. Razafsky, S. Zang, A. Giri, P.-H. Wu, J. Marchand, A. Celedon, C.M. Hale, S.X. Sun, D. Hodzic, and D. Wirtz, The distinct roles of the nucleus and nucleus-cytoskeleton connections in three-dimensional cell migration, in: Scientific Reports. Vol. 2; 488–499. 2014, D.H. Kim, S. Cho, and D. Wirtz, Tight coupling between nucleus and cell migration through the perinuclear actin cap, in: Journal of Cell Science. Vol. 127; 2528–2541. 2014, D. Razafsky, D. Wirtz, and D. Hodzic, Nuclear envelope in nuclear positioning and cell migration, in: Advances in Experimental Medicine and Biology. Vol. 773; 471–490. 2015, D.H. Kim and D. Wirtz, Cytoskeletal tension induces the 3D polarized architecture of the nucleus, in: Biomaterials. Vol. 48;