at this point, these two things are mutually exclusive. The advent of third-generation sequencing technology is expanding the limits of genomic research as the cost of generating high quality sequencing data is decreasing. The idea of using multiple sequencing technologies to facilitate genome assembly may become an idea of the past as the quality of long sequencing reads (hundreds or thousands of base pairs) approaches and exceeds the quality of current second generation sequencing reads. The computational difficulties that are encountered during genome assembly will also become a concept of the past as computation efficiency and performance increases. The development of more efficient sequencing algorithms and assembly programs is needed to develop more effective assembly approaches that can tandemly incorporate sequencing reads from multiple technologies. Many of the current limitations in genomic research revolve around the ability to produce large amounts of high quality sequencing data and to assemble entire genomes of organisms of interest. Developing more effective hybrid genome assembly strategies is taking the next step in advancing sequence assembly technology and these strategies are guaranteed to become more effective as more powerful technologies emerge. References External links Hybrid Error Correction and De Novo Assembly of Single-Molecule Sequencing Reads https://www.youtube.com/watch?v=IZdB7zKZ4TE Virtual Poster: Hybrid Genome Assembly of a Nocturnal Lemur https://www.youtube.com/watch?v=tKVGqpQ7_ms National Center for Biotechnology Information: Genome Assembly http://www.ncbi.nlm.nih.gov/projects/genome/assembly/assembly.shtml Bioinformatics