Colonies living in close proximity are territorial and aggressive toward one another. Argentine ants in their native South America also co-exist with many other species of ants, and do not attain the high population densities that characterize introduced populations. In a series of experiments, ants of the same colony were isolated and fed different diets. The hydrocarbons from the diet were eventually incorporated into the cuticle of the subjects. Those that had the same diet appeared to recognize one another as kin. Those who had at least some overlap in dietary composition also appeared to react non-aggressively to one another. These interactions contrasts drastically with the groups that fed on completely different sources, such as those who lived off flies and those that fed on grasshoppers. The groups appeared to have incorporated hydrocarbons that were not similar to the others and created an unfamiliar identity cue. These groups reacted violently towards each other. This suggests that dietary factors affect the recognition cues for colony members. Reproduction and seasonal colony trends Like workers in many other ant species, Argentine ant workers are unable to lay reproductive eggs but can direct the development of eggs into reproductive females; the production of males appears to be controlled by the amount of food available to the larvae. Argentine ant colonies almost invariably have many reproductive queens, as many as eight for every 1,000 workers. The seasonal low occurs in mid-winter, when 90% of a representative colony consists of workers and the remainder of queens, and no reproductive activity and minimal birthing. Eggs are produced in late-winter, nearly all of which hatch into sexual forms by May. Mating occurs after the females emerge. Worker production increases steadily from mid-March to October, after which their numbers are not replenished; thus, their numbers drop steadily over the winter months. Colonies in the Argentine ant's native habitat are kept within a range of ten to one hundred meters by colonies of interspecific and intraspecific rivals. As the colonies expand, they appear to form fluctuating territory borders, which contract and expand on a seasonal and conditional basis. There is an expansive push outward in the summer months, with a retreating motion in the winter. This has to do with soil moisture and temperature conditions. At the edges of these borders are either rival L. humile colonies or other obstacles that prevent further expansion, such as an inhospitable environment for