AOT may refer to: Science and technology Abstract object theory Aerosol OT, a common chemical reagent; see Dioctyl sodium sulfosuccinate Ahead-of-time compilation, an implementation approach for programming languages Assisted outpatient treatment, an implementation of mental-health law in the U.S. Transport Aeronautical operations technician Airports of Thailand, an airport operator Aosta Airport (IATA code), Italy Autorité organisatrice de transports, a French legal structure for the provision of public transport Oil transporter (US Navy hull classification symbol: AOT) Media Army of Two, a video game Attack on Titan, a Japanese manga series by Hajime Isayama Other uses Academy of Technology, an engineering college in West Bengal, India A.O. Trikala, a football club in Trikala, Greece