Anne d'Alençon (Italian: Anna d'Alençon) (30 October 1492 – 18 October 1562), Lady of La Guerche, was a French noblewoman and a Marquise of Montferrat as the wife of William IX, Marquis of Montferrat. She acted as Regent of the Marquisate of Montferrat for her son, Boniface from 1518 to his death in 1530. Life Anne was the third child of René, Duke of Alençon and his second wife Margaret of Lorraine, daughter of Frederick, Count of Vaudémont and Yolande of Anjou. Her father died two days after her birth. Marriage She was betrothed to William IX, Marquis of Montferrat of the Palaiologos family in 1501 and married him on 31 October 1508, the day after her sixteenth birthday, in the church of St. Sauveur in Blois. She bore her husband three children: Maria (1509–1530), Margherita, (1510–1566), and Boniface (1512–1530), William's heir who became Boniface IV of Montferrat. In 1517, her eldest daughter, Maria, was betrothed to Federico II Gonzaga, son of Isabella d'Este, who later became Marquis and Duke of Mantua. The marriage contract was annulled, however, after Federico accused Maria of attempting to poison his mistress Isabella Boschetti, wife of the Count of Calvisano. Regency On the death of William in 1518, the infant Boniface inherited the Marquisate. Anne, however, acted as regent until his unexpected death in June 1530. She remained involved in the government of Montferrat when Boniface was succeeded as Marquis by her brother-in-law John George, previously commendatory abbot of Lucedio and (unconsecrated) Bishop of Casale. Later life Boniface's death also rekindled Federico II Gonzaga's interest in marrying Maria. On the latter's unexpected death in September 1530 his attentions turned to the second daughter, Margherita. Having weighed up the various proposals for Margherita's hand, Anne opted for the link with the House of Gonzaga and the marriage was concluded in October 1531. In 1533 the Marquis John George died, leaving one natural son, but no heir. A dispute over the succession to the Marquisate, an imperial fiefdom, ensued, the contenders being the Gonzaga, the Marquis of Saluzzo and the House of Savoy, all of whom were able to make plausible claims. Meanwhile, the Montferrat was effectively under Spanish domination. In 1536 Charles V, Holy Roman Emperor assigned the fiefdom to Margherita Paleologa and her husband, Federico II, Duke of Mantua. Anne, however, returned as de facto ruler. In 1540 Federico II died at Marmirolo and