The Treaty of Tordesillas signed on November 15, 1524 ratified the treaty of Burgos signed on June 7, 1524, between the Lord of Monaco and Habsburg Spain. The treaty placed Monaco under the protection of Charles V, Holy Roman Emperor and King of Spain, as an imperial fief. As a consequence, the Lord of Monaco became subservient to Charles as his vassal and it was the beginning of the so-called Spanish protectorate over Monaco. Thus, the Lord of Monaco was required to give feudal homage, but Lucien I of Monaco requested that requirement be removed from the treaty and obtained for the final proclamation of November 1524 to recognize the autonomy of Monaco. Background: Monaco caught in the fire of the Italian Wars On August 22, 1523, the ruling Prince of Monaco, Lucien Grimaldi was assassinated by his cousin Barthélémy Doria, who stabbed him forty-two times. The population of Monaco intervened in favour of the Grimaldi dynasty and refused this coup. Barthélémy Doria who tried to escape his crime was caught and detained in the vicinity of La Turbie before being released by the House of Savoy. Meanwhile, Augustine Grimaldi, younger brother of Lucien and then bishop of Grasse, rose to the throne of Monaco as a regent in favour of his nephew Honoré by dispositions of his mother Claudine Grimaldi. Fearing for the sovereignty of his Principality, Augustine Grimaldi obtained from Pope Clement VII a papal bull promulgated on February 19, 1524, which consecrated the autonomy of Monaco. However, despite the support of French King François I, Augustine Grimaldi was blocked by the superseding influence of Andrea Doria at the Court of France, impeding him from prosecuting Barthélémy Doria for his crime. In order to obtain justice, Augustine Grimaldi obtained the vassality of Genovese territories under the influence of Andrea Doria and on November 3, 1523, the citizens of Dolceaqua came to pledge allegiance to the Grimaldi. Following the defeat of Guillaume Gouffier, seigneur de Bonnivet, at the battle of Gattinara in 1524, François I was losing influence in the area while Andra Doria's gallera plundered the Monegasque coast and Augustine Grimaldi was almost killed by the bombardments of these galleras in Menton. These events "forced" Augustineto pick up on the negotiations that his brother Lucien Grimaldi had begun with the lieutenants of Charles Quint. Augustine Grimaldi offered the port of Monaco to be used as a military basis