August 2010 in the non-peer reviewed online physics archive "arxiv.org" that they were able to have these cells "reproduce" when incubated at high pressure saturated steam at 121 °C (autoclaved) for up to two hours. Their conclusion is that these cells reproduced, without DNA, at temperatures higher than any known life form on earth is able to. They claimed that the cells, however, were unable to reproduce at temperatures similar to known organisms. Regarding the "absence" of DNA, Louis admits he has no training in biology, and has not reported the use of any standard microbiology growth medium to culture and induce germination and growth of the spores, basing his claim of "biological growth" on light absorption measurements following aggregation by supercritical fluids, an inert physical observation. Both his collaborators, Wickramasinghe and Milton Wainwright independently extracted and confirmed the presence of DNA from the spores. The absence of DNA was key to Louis and Kumar's hypothesis that the cells were of extraterrestrial origins. Louis' only reported attempt to stain the spores' DNA was by the use of malachite green, which is generally used to stain bacterial endospores, not algal spores, whose primary function of their cell wall and their impermeability is to ensure its own survival through periods of environmental stress. They are therefore resistant to ultraviolet and gamma radiation, desiccation, lysozyme, temperature, starvation and chemical disinfectants. Visualizing algal spore DNA under a light microscope can be difficult due to the impermeability of the highly resistant spore wall to dyes and stains used in normal staining procedures. The spores' DNA is tightly packed, encapsulated and desiccated, therefore, the spores must first be cultured in suitable growth medium and temperature to first induce germination, then cell growth followed by reproduction before staining the DNA. Other researchers have noted recurring instances of red rainfalls in 1818, 1846, 1872, 1880, 1896, and 1950 and several times since then. Most recently, coloured rainfall occurred over Kerala during the summers of 2001, 2006, 2007, 2008, and 2012; since 2001, the botanists have found the same Trentepohlia spores every time. This supports the notion that the red rain is a seasonal local environmental feature caused by algal spores. In popular culture The science fiction film Red Rain was loosely based on the red rain in Kerala story. It was directed by Rahul Sadasivan and released in India on 6 December 2013. See also References External links