can be retained and replayed for additional analysis Laser diffraction uses the principle that the angle of diffraction increases as particle size decreases, this method is used for measuring sizes between 0.1 and 3,000μm. Laser diffraction measures particle size distributions by concentration either as a percentage or by mass of a dispersed particulate sample. A Coulter counter is an apparatus for counting and sizing particles suspended in electrolytes. It is typically used for cellular particles. The Coulter principle, and the Coulter counter that is based on it, is the commercial term for the technique known as resistive pulse sensing or electrical zone sensing. Detection methods There are several methods used for detecting and measuring particle size or size distribution — light blocking (obscuration), light scattering, Coulter principle and direct imaging. A high intensity light source is used to illuminate the particle as it passes through the detection chamber. The light blocking optical particle counter method is typically useful for detecting and sizing particles greater than 1 micrometre in size and is based upon the amount of light a particle blocks when passing through the detection area of the particle counter. This type of technique allows high resolution and reliable measurement. If light scattering is used, then the redirected light is detected by a photo detector. The light scattering method is capable of detecting smaller-sized particles. This technique is based upon the amount of light that is deflected by a particle passing through the detection area of the particle counter. This deflection is called light scattering. Typical detection sensitivity of the light scattering method is 0.05 micrometre or larger. However, employment of the condensation nuclei counter (CNC) technique would allow a higher detection sensitivity in particle sizes down to nanometre range. A typical application is monitoring of ultrapure water in semiconductor fabrication facilities. If light blocking (obscuration) is used the loss of light is detected. The amplitude of the light scattered or light blocked is measured and the particle is counted and tabulated into standardized counting bins. The light blocking method is specified for particle counters that are used for counting in hydraulic and lubricating fluids. Particle counters are used here to measure contamination of hydraulic oil, and therefore allow the user to maintain their hydraulic system, reduce breakdowns, schedule maintenance during no or slow work periods, monitor filter performance, etc. Particle counters used for this purpose typically use ISO Standard