but Perrine soon found out that her husband's newfound calmness was temporary, only two months. The man's state of health and mind changed abruptly due to his binges of wine, tobacco, and ‘strong waters’ (alcohol). The man's madness was worse than before. Denys performed a second transfusion which diminished the delirium but induced other major side effects. The third and last transfusion performed on Mauroy happened under major pressure of the wife, in fact, Denys was against it. During the procedure Mauroy's body, at a certain point, shook in a ‘violent fit’ to which the men decided to end the transfusion. Mauroy died the next day. Recalling the various details of the transfusion it was clear that the cause of death had not been the transfusion, since no blood had actually been transfused into Mauroy and the calf had not yet been open when the seizures started. Denys and Emmerez tried to perform an autopsy but they were strongly opposed by the wife. The Trial Following Antoine Mauroy's death, a case was formed on April 17, 1668, and presented to the Court of Grand Châtelet. Denys was convinced that his transfusions were not causative of Mauroy's death, and that this trial was rather a consequence of his decision to pursue research against the will of the King's Academy of Sciences as well as that of the major players of the conservative Parisienne Faculty of Medicine. In an attempt to prove his innocence, Denys told Commissioner Le Cerf his story. He described his medical experiments and explained their safety, which was proved by the many survivors willing to witness in his favor. Finding sufficient grounds for concern, La Cerf forwarded the case to the Criminal Lieutenant, the Honorable Jacques Defita, for a full hearing. The witnesses at the trial included Perrine Mauroy, Mauroy's late wife, allegedly, persuaded and offered large amounts of money by several "unknown" physicians, to bear false witness and file reports against Denys’ blood transfusion experiments. Following a police investigation, vials with arsenic powder were found in Perrine Mauroy's possession. Arsenic poisoning was known to harm the nervous system and cause symptoms such as tremors, seizures and delirium, this could therefore explain Mauroy's intense delusional behavior prior to the third transfusion. It was therefore suspected that Perrine Mauroy had been administering arsenic powder to her husband's broth. Judge Defita cleared Denys of all accusations and Perrine was charged