Lake Ontario. It was also found in a walleye die-off in the landlocked inland Conesus Lake, the westernmost of the Finger Lakes in western New York state. This was the first case in the region outside waters contiguously connected to the Great Lakes. On May 12, 2007, the Wisconsin DNR announced the likely presence of VHS in Wisconsin's inland Little Lake Butte des Morts. Preliminary tests of samples of freshwater drum collected on May 2 were positive, and the announcement came amidst a die-off of hundreds of freshwater drum there and on neighboring Lake Winnebago. Preliminary tests later indicated VHS in specimens from Lake Winnebago. The lakes drain through the Fox River to Lake Michigan's Green Bay. On May 17, 2007, the Michigan DNR confirmed the presence of VHS in the Michigan's inland Budd Lake, a popular fishing destination is in the central part of Michigan's lower peninsula. A major die-off of VHS-positive muskies, bluegills, and black crappie began on April 30, 2007. On May 24, 2007, preliminary tests indicated the presence of VHS in a brown trout from Lake Michigan, the second largest freshwater lake in the United States. Contamination in the lake had been expected for months by experts, since the presence of VHS was confirmed in the connected Lake Huron. On July 14, 2007, federal labs confirmed the presence of VHS in Skaneateles Lake, the second of New York's Finger Lakes to test positive for the disease. The disease caused a large die-off of bass in the spring of 2007. Government regulation Before the Type IVb die-offs in the Great Lakes, few states regulated transfer of live species other than for salmonids. Since 2005, new policies have been adopted concerning fish and egg transfer, use of live bait, and water transfer, aimed at curtailing the spread to new lakes and rivers in the region. As of July 13, 2007, new rules have been enacted in the Canadian province of Ontario, and US states of Michigan, New York, Ohio, Pennsylvania, and Wisconsin, while they are currently being drafted in Illinois, Indiana, and Minnesota. Additionally, the USDA's Animal and Plant Health Inspection Service issued a federal order in the fall of 2006 barring the transfer of all live susceptible species from the eight states bordering the lower Great Lakes, as well as importing such species from the Canadian provinces of Ontario and Quebec. Transmission VHSV can be spread from