the sequential action of (ubiquitin-activating enzyme E1), several ubiquitin transferases and finally by [ubiquitin transferase RING E3 (calmodulin-selective)] EC 6.3.2.22: Now , diphthine—ammonia ligase. : homoglutathione synthase : tyrosine—arginine ligase : tubulin—tyrosine ligase : N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase EC 6.3.2.27: The activity is covered by two independent enzymes, N2-citryl-N6-acetyl-N6-hydroxylysine synthase, and , aerobactin synthase EC 6.3.2.28: Now , L-alanine-L-anticapsin ligase : cyanophycin synthase (L-aspartate-adding) : cyanophycin synthase (L-arginine-adding) : coenzyme F420-0:L-glutamate ligase : coenzyme γ-F420-2:α-L-glutamate ligase : tetrahydrosarcinapterin synthase : coenzyme F420-0:L-glutamate ligase : D-alanine—D-serine ligase : 4-phosphopantoate—β-alanine ligase : UDP-N-acetylmuramoyl-L-alanyl-D-glutamate—D-lysine ligase : N2-citryl-N6-acetyl-N6-hydroxylysine synthase : aerobactin synthase : cyclopeptine synthase * : N-acetylaspartylglutamate synthase * : synthase * : [amino-group carrier protein]—L-2-aminoadipate ligase * : pantoate—β-alanine ligase (ADP-forming) * : UDP-N-acetylmuramate—L-alanyl-γ-D-glutamyl-meso-2,6-diaminoheptanedioate ligase * : fumarate—(S)-2,3-diaminopropanoate ligase * : dapdiamide synthase * : L-arginine-specific L-amino acid ligase * : L-alanine—L-anticapsin ligase * : tenuazonic acid synthetase * : phosphopantothenate—cysteine ligase (ATP) * : jasmonoyl—L-amino acid ligase * : UDP-N-acetylmuramoyl-L-alanine—L-glutamate ligase * : L-2,3-diaminopropanoate—citrate ligase * : 2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase * : staphyloferrin B synthase * : staphyloferrin A synthase * : D-ornithine—citrate ligase * : 3-methyl-D-ornithine—L-lysine ligase * : glutamate—[amino group carrier protein] ligase * : tubulin-glutamate ligase * : β-tubulin-glutamate ligase * * No Wikipedia article EC 6.3.3: Cyclo-Ligases : phosphoribosylformylglycinamidine cyclo-ligase : 5-formyltetrahydrofolate cyclo-ligase : dethiobiotin synthase : (carboxyethyl)arginine β-lactam-synthase : O-ureido-D-serine cyclo-ligase * : carbapenam-3-carboxylate synthase * : Ni-sirohydrochlorin a,c-diamide reductive cyclase * * No Wikipedia article EC 6.3.4: Other Carbon-Nitrogen Ligases EC 6.3.4.1: Now included in , GMP synthase (glutamine-hydrolysing) : CTP synthase (glutamine hydrolysing) : formate—tetrahydrofolate ligase : adenylosuccinate synthase : argininosuccinate synthase : urea carboxylase : ribose-5-phosphate—ammonia ligase : imidazoleacetate—phosphoribosyldiphosphate ligase : biotin—[methylmalonyl-CoA-carboxytransferase] ligase : biotin—[methylmalonyl-CoA-carboxytransferase] ligase : biotin—[methylcrotonoyl-CoA-carboxylase] ligase : glutamate—methylamine ligase : phosphoribosylamine—glycine ligase : biotin carboxylase : biotin—[biotin carboxyl-carrier protein] ligase : carbamoyl-phosphate synthase (ammonia) : formate—dihydrofolate ligase : 5-(carboxyamino)imidazole ribonucleotide synthase : tRNAIle-lysidine synthase : 7-cyano-7-deazaguanine synthase : nicotinate phosphoribosyltransferase : tRNAIle2-agmatinylcytidine synthase * : formate—phosphoribosylaminoimidazolecarboxamide ligase * : tyramine—L-glutamate ligase * : 2-amino-2′-deoxyadenylo-succinate synthase * * No Wikipedia article EC 6.3.5: Carbon-nitrogen ligases with glutamine as amido-N-donor : NAD+ synthase (glutamine-hydrolysing) : GMP synthase (glutamine-hydrolysing) : phosphoribosylformylglycinamidine synthase : asparagine synthase (glutamine-hydrolysing) : carbamoyl-phosphate synthase (glutamine-hydrolysing) : asparaginyl-tRNA synthase (glutamine-hydrolysing) : glutaminyl-tRNA synthase (glutamine-hydrolysing) EC 6.3.5.8: Now , aminodeoxychorismate synthase. As ATP is not hydrolysed during the reaction, the classification of the enzyme as a ligase was incorrect : hydrogenobyrinic acid a,c-diamide