and pharmacological research on this plant have been reported. The extract was shown to have anti-inflammatory, anti-arthritic [6], antioxidant, and antidiabetic properties in vitro. A study conducted by Yanbei Tu in 2019, in which they examined the anti-arthritic impact of ethanol extract from Glycine tabacina on a collagen-induced arthritis mouse model. The mouse model orally received Glycine tabacina extract powder [1.11g, 2.22g, 4.44g] daily beginning from day zero and extended for 30 days. The extent of arthritis was determined by measuring the width of paws, arthritis index levels, X – rays, histopathological changes and swelling size. Physical examination on mouse models, swelling on paws and arthritis index revealed that orally administering Glycine tabacina extract substantially reduced arthritic symptoms in collagen – induced arthritis mouse models. Through X – ray and histopathological analyses, it was highlighted that Glycine tabacina extract actively shielded the cartilage and bone joints against deterioration, lesion, and distortion. In this study, to evaluate the anti-arthritic effect of ethanol extracted from G.tabacina (GTE), collagen-induced rats were used as a pathologic model. In the present day, it has been observed that numerous herds and natural extracts have the potential to have anti-arthritic having negligible toxicities as well as side effects. This species of plant has previously been used to treat diseases such as rheumatism and nephritis. However, little is known about the chemical constituents as well as the pharmacological activities of G.tabacina (GTE). At present, cytokines: TNF-α, IL-6, IL-1β and IL-1 are blocked using biological products having therapeutic capabilities such as Infliximab (anti-TNF-α Ab), Anakinra (IL-1R antagonist) and Tocilizumab (anti-IL-6R Ab). In this study, the severity of rheumatoid arthritis was determined by observing the serum levels of the cytokines: IL-1β, IL-6 and TNF-α on different days (14,21,28) which resulted in significantly decreased cytokine serum levels and validated the anti-arthritis activity of G.tabacina (GTE). In rheumatoid arthritis, oxidative stress is considered to play an important part in chronic inflammation which causes neutrophils residing in the joint compartment to produce increased amounts of reactive oxygen species (ROS) causing cartilage and bone damage. Further, the increased amounts of ROS in rheumatoid arthritis sufferers also caused lipid peroxidation and DNA deterioration as well as cause the retardation of defensive mechanisms (i.e., SOD and catalase). The cytokine: TNF-α plays a major role in regulating rheumatoid arthritis activity and worsens the inflammation via the production of ROS when oxidative stress is heightened. This study observed