Cypovirus, short for cytoplasmic polyhedrosis virus, is a genus of double-stranded RNA viruses in the family Reoviridae and subfamily Spinareovirinae. Cypoviruses have only been isolated from insects. Diseases associated with this genus include chronic diarrhoea and pale blue iridescence in the guts of larvae. Sixteen species are placed in this genus. Cypoviruses structurally have muc in common with the more widely studied nucleopolyhedroviruses, a genus of arthropod viruses in the family Baculoviridae. However, cypoviruses have an RNA genome and replicate in the cytoplasm of the infected cells, while nucleopolyhedroviruses have a DNA genome and replicate in the nucleus. Structure Viruses in the genus Cypovirus are nonenveloped, with icosahedral geometries, and T=2 symmetry. Cypoviruses have only a single capsid shell, which is similar to the orthoreovirus inner core. They exhibit striking capsid stability, which is fully capable of endogenous RNA transcription and processing. The capsid diameter is around 65 nm. Genome Genomes are linear and segmented. The genome codes for 10 to 12 proteins. Classification of cypoviruses is based on the electrophoretic migration profiles of their genome segments. The overall folds of cypovirus proteins are similar to those of other reoviruses, but they have insertional domains and unique structures that contribute to their extensive intermolecular interactions. The cypovirus turret protein contains two methylase domains with a highly conserved helix-pair/β-sheet/helix-pair sandwich fold, but lacks the β-barrel flap present in orthoreovirus λ2. The stacking of turret protein functional domains and the presence of constrictions and A spikes along the mRNA release pathway indicate a mechanism that uses pores and channels to regulate the highly coordinated steps of RNA transcription, processing, and release. Lifecycle Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment to host receptors, which mediates endocytosis. Replication follows the double-stranded RNA virus replication model. Double-stranded RNA virus transcription is the method of transcription. Translation takes place by ribosomal skipping. The virus exits the host cell by monopartite nontubule guided viral movement, and exists in occlusion bodies after cell death and remains infectious until finding another host. Insects serve as their natural hosts. Transmission routes are fecal-oral, parental, and egg transmission. Infection occurs when a susceptible insect consumes viral polyhedra, usually as a contaminant on the insect’s food (in most cases, foliage of a plant). The polyhedra dissolve in the digestive tract of the insect, releasing the virus particles that penetrate the gut epithelial cells. Replication of the