the upper atmosphere of Titan. On July 26, 2017, Cassini scientists positively identified the presence of carbon chain anions in Titan's upper atmosphere which appeared to be involved in the production of large complex organics. These highly reactive molecules were previously known to contribute to building complex organics in the Interstellar Medium, therefore highlighting a possibly universal stepping stone to producing complex organic material. On July 28, 2017, scientists reported that acrylonitrile, or vinyl cyanide, (C2H3CN), possibly essential for life by being related to cell membrane and vesicle structure formation, had been found on Titan. In October 2018, researchers reported low-temperature chemical pathways from simple organic compounds to complex polycyclic aromatic hydrocarbon (PAH) chemicals. Such chemical pathways may help explain the presence of PAHs in the low-temperature atmosphere of Titan, and may be significant pathways, in terms of the PAH world hypothesis, in producing precursors to biochemicals related to life as we know it. Possible subsurface habitats Laboratory simulations have led to the suggestion that enough organic material exists on Titan to start a chemical evolution analogous to what is thought to have started life on Earth. The analogy assumes the presence of liquid water for longer periods than is currently observable; several hypotheses postulate that liquid water from an impact could be preserved under a frozen isolation layer. It has also been hypothesized that liquid-ammonia oceans could exist deep below the surface. Another model suggests an ammonia–water solution as much as deep beneath a water-ice crust with conditions that, although extreme by terrestrial standards, are such that life could survive. Heat transfer between the interior and upper layers would be critical in sustaining any subsurface oceanic life. Detection of microbial life on Titan would depend on its biogenic effects, with the atmospheric methane and nitrogen examined. Methane and life at the surface It has been speculated that life could exist in the lakes of liquid methane on Titan, just as organisms on Earth live in water. Such organisms would inhale H2 in place of O2, metabolize it with acetylene instead of glucose, and exhale methane instead of carbon dioxide. However, such hypothetical organisms would be required to metabolize at a deep freeze temperature of . All life forms on Earth (including methanogens) use liquid water as a solvent; it is speculated that life on Titan might instead use a liquid hydrocarbon, such as methane or ethane, although water is