need for intensive tillage under crop rotation allows biomass aggregation to lead to greater nutrient retention and utilization, decreasing the need for added nutrients. With tillage, disruption and oxidation of soil creates a less conducive environment for diversity and proliferation of microorganisms in the soil. These microorganisms are what make nutrients available to plants. So, where "active" soil organic matter is a key to productive soil, soil with low microbial activity provides significantly fewer nutrients to plants; this is true even though the quantity of biomass left in the soil may be the same. Soil microorganisms also decrease pathogen and pest activity through competition. In addition, plants produce root exudates and other chemicals which manipulate their soil environment as well as their weed environment. Thus rotation allows increased yields from nutrient availability but also alleviation of allelopathy and competitive weed environments. Carbon sequestration Studies have shown that crop rotations greatly increase soil organic carbon (SOC) content, the main constituent of soil organic matter. Carbon, along with hydrogen and oxygen, is a macronutrient for plants. Highly diverse rotations spanning long periods of time have shown to be even more effective in increasing SOC, while soil disturbances (e.g. from tillage) are responsible for exponential decline in SOC levels. In Brazil, conversion to no-till methods combined with intensive crop rotations has been shown an SOC sequestration rate of 0.41 tonnes per hectare per year. In addition to enhancing crop productivity, sequestration of atmospheric carbon has great implications in reducing rates of climate change by removing carbon dioxide from the air. Nitrogen fixing Rotating crops adds nutrients to the soil. Legumes, plants of the family Fabaceae, for instance, have nodules on their roots which contain nitrogen-fixing bacteria called rhizobia. During a process called nodulation, the rhizobia bacteria use nutrients and water provided by the plant to convert atmospheric nitrogen into ammonia, which is then converted into an organic compound that the plant can use as its nitrogen source. It therefore makes good sense agriculturally to alternate them with cereals (family Poaceae) and other plants that require nitrates. How much nitrogen made available to the plants depends on factors such as the kind of legume, the effectiveness of rhizobia bacteria, soil conditions, and the availability of elements necessary for plant food. Pathogen and pest control Crop rotation is also used to control pests and diseases that can become established in the soil over time. The