already missing when Wilfrid Voynich acquired the manuscript in 1912. There is strong evidence that many of the book's bifolios were reordered at various points in its history, and that the original page order may well have been quite different from what it is today. Parchment, covers, and binding Samples from various parts of the manuscript were radiocarbon dated at the University of Arizona in 2009. The results were consistent for all samples tested and indicated a date for the parchment between 1404 and 1438. Protein testing in 2014 revealed that the parchment was made from calf skin, and multispectral analysis showed that it had not been written on before the manuscript was created (i.e., it is not a palimpsest). The quality of the parchment is average and has deficiencies, such as holes and tears, common in parchment codices, but was also prepared with so much care that the skin side is largely indistinguishable from the flesh side. The parchment is prepared from "at least fourteen or fifteen entire calfskins". Some folios (e.g., 42 and 47) are thicker than the usual parchment. The goat skin binding and covers are not original to the book, but date to its possession by the Collegio Romano. Insect holes are present on the first and last folios of the manuscript in the current order and suggest that a wooden cover was present before the later covers. Discolouring on the edges points to a tanned-leather inside cover. Ink Many pages contain substantial drawings or charts which are colored with paint. Based on modern analysis using polarized light microscopy (PLM), it has been determined that a quill pen and iron gall ink were used for the text and figure outlines. The ink of the drawings, text, and page and quire numbers have similar microscopic characteristics. In 2009, energy-dispersive X-ray spectroscopy (EDS) revealed that the inks contained major amounts of carbon, iron, sulfur, potassium and calcium with trace amounts of copper and occasionally zinc. EDS did not show the presence of lead, while X-ray diffraction (XRD) identified potassium lead oxide, potassium hydrogen sulphate, and syngenite in one of the samples tested. The similarity between the drawing inks and text inks suggested a contemporaneous origin. Paint Colored paint was applied (somewhat crudely) to the ink outlined figures, possibly at a later date. The blue, white, red-brown, and green paints of the manuscript have been analyzed using PLM, XRD,