and the software MaxMSP define middle C as C3. Apple's GarageBand also defines middle C (261.6256 Hz) as C3. Using scientific pitch notation consistently, the MIDI NoteOn message assigns MIDI note 0 to C−1 (five octaves below C4 or Middle C; lowest note on the two largest organs of the world; about one octave below the human hearing threshold: its overtones, however, are audible), MIDI note 21 to A0 (the bottom key of an 88-key piano), MIDI note 60 to C4 (Middle C), MIDI note 69 to A4 (A440), MIDI note 108 to C8 (the top key of an 88-key piano), and MIDI note 127 to G9 (beyond the piano; one octave above the highest note on some keyboard glockenspiels; some notes above the highest-pitched organ pipes). This creates a linear pitch space in which an octave spans 12 semitones, where each semitone is the distance between adjacent keys of the piano keyboard. Distance in this space corresponds to musical pitch distance in an equal-tempered scale, 2 semitones being a whole step, and 1 semitone being a half step. An equal-tempered semitone can also be subdivided further into 100 cents. Each cent is semitone or octave. This measure of pitch allows the expression of microtones not found on standard piano keyboards. Meantone temperament The notation is sometimes used in the context of meantone temperament, and does not always assume equal temperament nor the standard concert A4 of 440 Hz; this is particularly the case in connection with earlier music. The standard proposed to the Acoustical Society of America explicitly states a logarithmic scale for frequency, which excludes meantone temperament, and the base frequency it uses gives A4 a frequency of exactly 440 Hz. However, when dealing with earlier music that did not use equal temperament, it is understandably easier to simply refer to notes by their closest modern equivalent, as opposed to specifying the difference using cents every time. Table of note frequencies The table below gives notation for pitches based on standard piano key frequencies: standard concert pitch and twelve-tone equal temperament. When a piano is tuned to just intonation, C4 refers to the same key on the keyboard, but a slightly different frequency. Keys which do not appear on any piano (medium gray) or only on an extended 108-key piano (light gray) are highlighted. Mathematically, given the number of semitones above middle C, the fundamental frequency in hertz