protein. The bioinformatics analysis is facilitated by the bioinformatic search engine Harvester. 5. Assignment of subcellular localization category Results from the N- and C-terminal fusions are assessed and in turn these data are compared to the bioinformatic predictions. A final subcellular localisation (from approximately 20 categories) is then assigned for each ORF. Similar localisations with both N- and C-terminal constructs provide a higher degree of reliability of the result. For those ORFs where the two fusions do not give a similar localisation pattern, a series of other criteria, including bioinformatic predictions, are considered. Occasionally a clear cut localization cannot be assigned. Which data is published? Every data sheet contains the fluorescence images of both N- and C-terminal fusions, the assigned localization, other localizations, comments and the Swissprot ID. For every protein entry, a link is provided to the corresponding Harvester bioinformatics page. How do I use the GFP-cDNA database? Images of all localised proteins and their bioinformatic analysis can be viewed via the ‘Results Table’ or ‘Results Images’ buttons. In addition, use the search window on the entry site to find proteins containing features or motifs of particular interest to you that have been localised in this project. External links GFP-cDNA database FAQ Harvester bioinformatic search engine Sources Homepage of the GFP-cDNA project Bioinformatics