fuzzy system model and computer simulation. When new coefficients are introduced, their values can be fitted into the set. Related techniques Förster resonance energy transfer (FRET): 10 nm proximity (Light microscopy: only 250 nm resolution; no certainty of effective interaction) Immuno precipitation (IP) dropdowns / pulldowns Yeast 2 hybrid - protein interaction mapping Benchmark images The degree of colocalization in fluorescence microscopy images can be validated using the Colocalization Benchmark Source, a free collection of downloadable image sets with pre-defined values of colocalization. Software implementations open source FIJI is just ImageJ - batteries included BioImage XD closed source AxioVision Colocalization Module Colocalization Research Software CoLocalizer Pro CoLocalizer Pro Nikon's NIS-Elements Colocalization Module Scientific Volume Imaging's Huygens Colocalization Analyzer Quorum Technology's Volocity Media Cybernetics's Image-Pro Bitplane's Imaris arivis Vision4D References Microscopy