revealed a small genetic Northwest African contribution among today's inhabitants near the region of Lucera. During Spanish rule of Sicily During Spanish rule of Sicily, and to escape the Spanish inquisition of the Moriscos (Muslims who had converted to Christianity) in the Iberian peninsula, a few Moriscos migrated to Sicily. During this time there were several attempts to rid Sicily of its extensive formerly Muslim 'Moor' population. The attacks were also directed against crypto-Muslim slaves and Sicilian renegades who refused to deny Islam during the 16th and the 17th centuries. However, it is doubtful that the order was carried out in practice. The main reason that some former Muslims were able to remain in Sicily was that they were openly supported by The Duke of Osuna, now officially installed as viceroy in Palermo, advocated to the Spanish monarch in Madrid for allowing the Moriscos to stay in Sicily. 15th century: Ottomans in Otranto During this century, the Ottoman Empire was expanding mightily in southeastern Europe. It completed the absorption of the Byzantine Empire in 1453 under Sultan Mehmet II by conquering Constantinople and Galata. It seized Genoa's last bastions in the Black Sea in 1475 and Venice's Greek colony of Euboea in 1479. Turkish troops invaded the Friuli region in northeastern Italy in 1479 and again in 1499–1503. The Apulian harbor town of Otranto, located about 100 kilometers southeast of Brindisi, was seized in 1480 (Ottoman invasion of Otranto), but the Turks were routed there in 1481 by an alliance of several Italian city-states, Hungary and France led by the prince Alphonso II of Naples, when Mehmet died and a war for his succession broke out. Cem Sultan, pretender to the Ottoman throne, was defeated despite being supported by the pope; he fled with his family to the Kingdom of Naples, where his male descendants were bestowed with the title of Principe de Sayd by the Pope in 1492. They lived in Naples until the 17th century and in Sicily until 1668 before relocating to Malta. Attacks in the 16th century It is a subject of debate whether Otranto was meant to be the base for further conquests. In any case, the Ottoman sultans had not given up their ambition to take over the Italian Peninsula and to finally install Islamic sovereignty after the conquest of Constantinople. After the conquests of Ragusa (Dubrovnik) and Hungary in 1526 and the defeat