Sabine River on the east and the San Jacinto River on the west including much of Montgomery, Newton, Trinity, and Walker Counties, as well. Several attempts to define the boundaries of the Big Thicket have been made, including a biological survey in 1936 which included over covering 14 counties. A later botanical based study in 1972 included a region of over . Geography Physical geography Geology: What makes the Big Thicket a remarkable place is the unusually high diversity of plants concentrated in a relatively small area. A major factor enabling this diversity is an unusually high diversity of soils. The exposed surface soils are relatively recent, late Cenozoic Era, predominantly Miocene, Pliocene, Pleistocene, and Holocene Epoch formations. Other than some slight and uniform tilting, the strata are not greatly deformed. The strata were laid down in several cycles of glaciations and warmer interglacial interludes. Although glaciers never extended as far south as Texas, it was during these cold periods that enough water was frozen in polar regions to lower sea levels and the Big Thicket area was dry land well above sea level. Conversely, during the warmer interludes, enough ice melted to increase sea levels and submerge Southeast Texas in the sea. It was during those warmer periods of submersion that alluvial and delta deposits from the streams and rivers of the continent laid down the succession of strata, each new layer burying and compacting the previous. At some point there was an approximately 1% tilting of the layers. Some say it was caused by the rising of the Rocky Mountains, while others say it was the weight of the successive deposits that eventually caused the Gulf of Mexico to subside. The older Pliocene, Miocene, and Oligocene formations are exposed in the north (Polk, San Jacinto, and Tyler counties), while the more recent Quaternary Period formations are exposed in the south (Hardin and Liberty counties). Soils: The soils of the various formations at the surface have shifted and intermingled through erosion and other factors over time and can be locally quite complex. The Big Thicket contains a greater variety of soils than any area of comparable size in the United States. Sources vary, but from 50 to 100 soil types are said to occur in Hardin County alone. The surface soils tend to be slightly to strongly acidic, and only occasionally neutral in much of the Big Thicket Basin.