Near-infrared spectroscopy (NIRS) is a spectroscopic method that uses the near-infrared region of the electromagnetic spectrum (from 780 nm to 2500 nm). Typical applications include medical and physiological diagnostics and research including blood sugar, pulse oximetry, functional neuroimaging, sports medicine, elite sports training, ergonomics, rehabilitation, neonatal research, brain computer interface, urology (bladder contraction), and neurology (neurovascular coupling). There are also applications in other areas as well such as pharmaceutical, food and agrochemical quality control, atmospheric chemistry, combustion research and knowledge Theory Near-infrared spectroscopy is based on molecular overtone and combination vibrations. Such transitions are forbidden by the selection rules of quantum mechanics. As a result, the molar absorptivity in the near-IR region is typically quite small. (NIR absorption bands are typically 10–100 times weaker than the corresponding fundamental mid-IR absorption band.) One advantage is that NIR can typically penetrate much further into a sample than mid infrared radiation. Near-infrared spectroscopy is, therefore, not a particularly sensitive technique, but it can be very useful in probing bulk material with little or no sample preparation. The molecular overtone and combination bands seen in the near-IR are typically very broad, leading to complex spectra; it can be difficult to assign specific features to specific chemical components. Multivariate (multiple variables) calibration techniques (e.g., principal components analysis, partial least squares, or artificial neural networks) are often employed to extract the desired chemical information. Careful development of a set of calibration samples and application of multivariate calibration techniques is essential for near-infrared analytical methods. History The discovery of near-infrared energy is ascribed to William Herschel in the 19th century, but the first industrial application began in the 1950s. In the first applications, NIRS was used only as an add-on unit to other optical devices that used other wavelengths such as ultraviolet (UV), visible (Vis), or mid-infrared (MIR) spectrometers. In the 1980s, a single-unit, stand-alone NIRS system was made available. In the 1980s, Karl Norris (while working at the USDA Instrumentation Research Laboratory, Beltsville, USA) pioneered the use NIR spectroscopy for quality assessments of agricultural products. Since then, use has expanded from food and agricultural to chemical, polymer, and petroleum industries; pharmaceutical industry; biomedical sciences; and environmental analysis. With the introduction of light-fiber optics in the mid-1980s and the monochromator-detector developments in the early 1990s, NIRS became a more powerful tool for scientific research. The method has been used in a number of fields of science including