The Avenue of the Saints is a highway in the Midwestern United States that connects St. Louis, Missouri and St. Paul, Minnesota. Route description Missouri The southern end of the Avenue of the Saints is at exit 28A of Interstate 64 (I‑64) /U.S. Route 40 (US 40), which is also the southern end of its concurrency with US 61. The Avenue of the Saints heads west, to Chesterfield, crossing I-270. Here, the Avenue of the Saints turns northwest, crosses the Missouri River via the Daniel Boone Bridge. In Wentzville, the route interchanges with I-70. The western end of I‑64 and the northern end of the concurrency with US 40 is here. The Avenue of the Saints continues north as just US 61, intersects with the western end of I-72 in Hannibal, reaches the northern terminus of its concurrency with US 61, then crosses the Des Moines River into Iowa. The Avenue of the Saints is co-signed with Missouri Route 27 to the Iowa state line. Iowa In Iowa, the Avenue of the Saints is a highway, which begins in Lee County where Missouri Route 27 crosses the Des Moines River, and ends at the Minnesota state line in Worth County, concurrent with Interstate 35. Construction of the Avenue of the Saints corridor in Iowa was completed on May 23, 2006. The route runs north from the Missouri state line as just Iowa Highway 27 for about , then is co-signed with US 218 until just south of Cedar Rapids, crossing I-80 in Iowa City. This is also the location of the southern end of its concurrency with I‑380, and this concurrency terminates in Waterloo. Near Floyd, US 218 splits off, just after the route starts a concurrency with US 18, running west to its intersection with I-35, where the route turns north again and runs into Minnesota. In 2001, the Iowa Department of Transportation designated the Avenue of the Saints as Iowa 27. Prior to its creation, motorists wanting to travel the Avenue through Iowa had to follow a lengthy list of directions: (heading north from Missouri) Iowa 394, US 218, I‑380, US 20, Iowa 58, US 218 (again), US 18 and I‑35. As with many rural expressways in Iowa, the Avenue of the Saints has exit numbers in that state. The exit numbers correspond to the underlying U.S. Highway or Interstate Highway – US 218, I-380, US 20, US