and Bovet, in the laboratory of Fourneau, paved the way for the sulfamidotherapy. The Hospital Pasteur The Hospital Pasteur was built during the first years of the 20th century in front of the institute and was employed for a long time by the members as a field for clinical observation and experimentations of therapeutical processes elaborated by themselves. Since in the beginning it was provided with only 120 beds, every patient was so well isolated in his private room that each room could be almost considered a small pest house, ideal for quarantine. The construction of the hospital was enabled by the gift of a benefactor, Madame Lebaudy, while money offered by the baroness Hirsch was used to build a large pavilion that accommodated the Department of Chemical Biology of the institute. Duclaux work in the chemical biology department The work done in the new pavilion by Duclaux clarified how the human body accomplished some of its vital functions and discovered the role of a diastase. It was critical in resolving a controversy aroused between Pasteur and Berthelot after the publication of Claude Bernard’s posthumous essay regarding the nature of the agents implicated in some transformations that happen inside plants, like fermentation. While Pasteur believed that the only substance implied in the process of fermentation was yeast, Bernard – and Berthelot in his own way – believed that some other soluble ferment was involved: the German chemist Eduard Buchner later demonstrated the existence of this "ferment", an intracellular diastase which he called "zymase," what we know now as enzymes. Duclaux's study on the metabolism of nutrients did not have immediate practical applications, but later revealed how extensive is the field of enzymes and opened new roads that would lead biology to extend the knowledge on life's mechanisms on a molecular level. Pasteur's museum and tomb The Musée Pasteur (Pasteur Museum) is located in the south wing of the first building occupied by the Institut Pasteur, which was inaugurated on 14 November 1888. Established in 1936, this museum preserves the memory of Louis Pasteur's life and work in the vast apartment where he lived during the last seven years of his life, from 1888 to 1895. This museum also includes the collection of scientific objects illustrating the scientist's work, as well as the Neo-Byzantine funeral chapel where Pasteur is buried. List of international locations Today, the Institut Pasteur houses 100 research