Aragon, by whom he had a daughter named Mary. His second marriage, to Anne Boleyn, resulted in a daughter named Elizabeth. Henry VIII had a son, Edward, by his third wife, Jane Seymour. An Act of Parliament passed in 1533 declared Mary illegitimate; another passed in 1536 did the same for Elizabeth. Though the two remained illegitimate, an Act of Parliament passed in 1544 allowed reinserting them, providing further "that the King should and might give, will, limit, assign, appoint or dispose the said imperial Crown and the other premises … by letters patent or last will in writing." Mary and Elizabeth, under Henry VIII's will, were to be followed by the granddaughters of the King's deceased sister Mary Tudor, Duchess of Suffolk (he, however, excluded Mary’s still living daughters, Frances Grey, Duchess of Suffolk and Eleanor Clifford, Countess of Cumberland). This will also excluded from the succession the descendants of Henry's eldest sister Margaret Tudor, who were the rulers of Scotland. When Henry VIII died in 1547, the young Edward succeeded him, becoming Edward VI. Edward VI was the first Protestant Sovereign to succeed to the throne of England. He attempted to divert the course of succession in his will to prevent his Catholic half-sister, Mary, from inheriting the throne. He excluded Mary and Elizabeth, settling on the Duchess of Suffolk's daughter, Lady Jane Grey. Jane was also originally excluded on the premise that no woman could reign over England. Nonetheless, the will, which originally referred to Jane's heirs-male, was amended to refer to Jane and her heirs-male. Upon Edward VI's death in 1553, Jane was proclaimed Queen of England and Ireland. She was not universally recognised and after nine days she was overthrown by the popular Mary. As Henry VIII's will had been approved by an act of Parliament in 1544, Edward's contravening will was unlawful and ignored. Mary was succeeded by her half-sister, Elizabeth, who broke with the precedents of many of her predecessors, and refused to name an heir. Whilst previous monarchs (including Henry VIII) had specifically been granted authority to settle uncertain successions in their wills, the Treasons Act 1571 asserted that Parliament had the right to settle disputes, and made it treason to deny Parliamentary authority. Wary of threats from other possible heirs, Parliament further passed the Act of Association 1584, which provided that any individual involved in attempts to murder the Sovereign would